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描述CD3+/CD20+犬类大细胞淋巴瘤的特征

Cory M Howard^1,2, Steffanie Anderson², Bonnie Harrington³

¹Department of Pathobiology and Diagnostic Investigation, Michigan State University College of Veterinary Medicine, East Lansing, MI, USA.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

|October 14, 2023

概括

具有T细胞 (CD3) 和B细胞 (CD20) 标记的犬类大细胞淋巴瘤 (LCL) 显示T细胞受体玛重组,类似于外围T细胞淋巴瘤 (PTCL). 这表明这些犬类淋巴瘤类型的共同起源.

科学领域:

兽医病理学兽医病理学
狗类瘤学狗类瘤学
分子诊断学分子诊断学

背景情况:

狗大细胞淋巴瘤 (LCL) 的免疫类型鉴定有助于预测临床结果.
CD3表达通常表明T细胞起源,而CD20标志着B细胞.
狗类LCL的一个子集共同表达CD3和CD20 (CD3+/CD20+),其分子特征尚不清楚.

研究的目的:

为了研究犬类CD3+/CD20+LCL.CL的免疫表型特性和抗原受体克隆性.
将这些特征与外围T细胞淋巴瘤 (PTCL) 和扩散大B细胞淋巴瘤 (DLBCL) 进行比较.

主要方法:

来自10个CD3+/CD20+LCL病例和对照组的甲固定,嵌组织的回顾性分析.
聚合酶链反应用于抗原受体重组 (PARR),以检测T细胞受体玛 (TCRγ) 和免疫球蛋白重链 (IgH) 的重组.
对B细胞标记物 (PAX5),BCL-2和增殖标记物 (Ki67) 的免疫组织化学.

主要成果:

所有CD3+/CD20+LCL病例都表现出单克隆TCRγ重排.
三例病例显示单克隆IgH重组,表明发生了跨血统事件.
CD3+/CD20+LCL与PTCL共享频繁的TCRγ重新排列,但与DLBCL相比,IgH重新排列的频率较低.
在CD3+/CD20+LCL中PAX5表达低于DLBCL;BCL-2表达一致.

结论:

狗中CD3+/CD20+LCL病例显示出主要类似PTCL的分子和免疫类型特性.
这些发现表明,狗的CD3+/CD20+LCL和PTCL具有相似的基因组发生.

关键词:

在BCL-2中.CD20 CD20 是一个CD20 CD20 是一个CD20.CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD3 CD4 CD4 CD4 CD4 CD4 CD4 CD5 CD5 CD5 CD5 CD5 CD6 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 CD7 DLBCL DLBCL 是一个字母.帕尔·帕尔 (Parr Parr) 是一个名字.在PAX5中使用PAX5.在PTCL中,PTCL是什么?狗类犬类动物狗类犬类大细胞淋巴瘤的大细胞淋巴瘤

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