CD95L结合体突出显示了CD95介导的细胞亡和非细胞亡通路的不同静态度
Eden Lebrault1, Christelle Oblet1, Keerthi Kurma2
1UMR CNRS 7276, INSERM U1262, CRIBL, Université Limoges, Limoges, France.
European journal of immunology
|October 14, 2023
概括
CD95L连接体结构决定了细胞的信号传递. 双相CD95L触发了亡,而三相和六相形式是反应所需的,揭示了不同的信号通路.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 免疫学 免疫学 免疫学
背景情况:
- CD95受体 (也称为Fas) 在调节亡中起着至关重要的作用.
- 结合CD95的CD95连接体 (CD95L) 诱导细胞反应,包括编程细胞死亡.
- 不同的信号结果所需的CD95L精确静脉测量仍然不完全理解.
研究的目的:
- 调查CD95L多元化在触发apoptotic和非apoptotic信号中的作用.
- 为了确定特定细胞反应所需的最低CD95L静脉测量.
- 阐明CD95L聚合,构造和下游信号通路之间的关系.
主要方法:
- 使用灵活的链接器生成具有不同静态度的CD95L结合体 (二分体到六分体).
- 评估 CD95 敏感的 Jurkat 细胞在用不同的 CD95L 形式刺激时的亡反应.
- 在对CD95L刺激的反应中测量细胞内 (Ca2+) 流量.
主要成果:
- 在诱导细胞死亡方面,CD95L六合体是最有效的,但二合体足以启动细胞亡反应.
- 只有三元和六元的CD95L形式引起了强烈的Ca2+反应.
- 亡信号似乎主要由CD95L聚合控制,而Ca2+信号需要聚合和特定形状.
结论:
- CD95L静脉测量是其信号容量的关键决定因素.
- 不同的CD95L多重体状态差异调节了apoptotic与Ca2+信号通路的对比.
- 了解CD95L结构-功能关系是解读其在细胞命运决定中的复杂作用的关键.
更多相关视频
09:18Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
Published on: December 27, 2016
8.7K
12:55Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
Published on: February 16, 2015
18.1K
相关概念视频
01:17The Extrinsic Apoptotic Pathway
6.4K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
01:31The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
01:30Apoptosis
11.5K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
11.5K
01:17Phagocytosis of Apoptotic Cells
3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.9K
01:24Caspases
12.5K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
12.5K
01:18Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K