Hsa-miR-877-5p在急性缺血性中风中的表达基于生物信息学分析和临床验证
Si-Shuo Zhang1, Ji-Wei Zhang2, Kai-Xin Zhang3
1Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jingshi Road No.16369 in Lixia District, Jinan, China.
Molecular neurobiology
|October 14, 2023
概括
研究人员将hsa-miR-877-5p确定为急性缺血性中风 (AIS) 的关键调节剂. 这种微RNA及其点IL-23,CXCR3和TNF-α显示出作为AIS新型治疗点的潜力.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
背景情况:
- 炎症和免疫在缺血性中风的发病过程中至关重要.
- 了解调节基因对于新型急性缺血性中风 (AIS) 的诊断和治疗目标至关重要.
研究的目的:
- 确定AIS中的关键调节基因和机制.
- 基于分子途径,探索AIS的潜在治疗点.
主要方法:
- 使用GEO数据库对AIS患者mRNA和miRNA的基因表达差异分析与健康对照.
- 构建一个miRNA-mRNA调节网络.
- 通过LASSO回归和SVM-RFE模型选关键miRNAs.
- 使用CIBERSORT和免疫试验对miRNA与免疫细胞和炎症因素的相关性分析.
- 在临床样本中通过定量PCR和ELISA验证.
主要成果:
- 鉴定出hsa-miR-877-5p是AIS中关键的调节性miRNA,调节免疫和炎症反应.
- 与健康个体相比,AIS患者的血液中hsa-miR-877-5p的表达显著更高.
- 在AIS患者中观察到高水平的炎症因子IL-23和TNF-α,以及IL-23,CXCR3和TNF-α的mRNA表达.
结论:
- hsa-miR-877-5p及其下游目标 (IL-23,CXCR3,TNF-α) 是AIS的潜在生物标志物和治疗目标.
- 这项研究提供了对AIS背后的分子机制的见解,强调了免疫和炎症通路的作用.
关键词:
急性缺血性中风是一次急性缺血性中风.生物信息学分析CXCR3CXCR3CXCR3CXCR3CXCR3CXCR3CXCR3CXCR3C它们有IL-23和IL-23.这就是TNF-α.这是一个hsa-miR-877-5pp.更多相关视频
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