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在盐应激和补充下对狐尾小米进行计算和实验分析

Seerat Saleem¹, Khalid M Alghamdi², Naveed Ul Mushtaq¹

¹Department of Bioresources, School of Biological Sciences, University of Kashmir, Srinagar, 190006, India.

Environmental science and pollution research international

|October 14, 2023

概括

(Se) 补充剂通过增强亚酸过氧化酶 (APX) 酶活性和基因表达来减轻狐尾小米的盐度 (NaCl) 压力. 低度的Se有效地减少氧化损伤,并改善盐应激下植物的生长.

科学领域:

植物科学植物科学
生物化学生化学
分子生物学分子生物学

背景情况:

盐度压力显著阻碍了作物生产率,构成了全球性挑战.
狐尾小米表现出对干旱和盐度等环境压力因素的固有耐受性.
亚酸盐过氧化酶 (APX) 对于排毒活性氧物种 (ROS) 和在压力下保持细胞稳定性至关重要.

研究的目的:

为了研究化 (NaCl) 和 (Se) 对狐尾小米中APX酶活性的影响.
阐明度应激的Se介导缓解背后的分子机制.
评估Se作为外部缓解剂的有效性,以提高狐尾小米的盐分耐受性.

主要方法:

在基分子对接和APX酶的3D结构建模.
在体外分析形态和生化参数.
在压力和治疗条件下评估基因调节的mRNA表达研究.

主要成果:

在分析表明Se和APX之间的相互作用比NaCl更强.
它的应用,特别是在较低度 (1μM),缓解了NaCl诱导的压力症状.
它增强了形态参数,上调了APX基因表达和酶活性,并降低了过氧化 (H2O2) 水平.
转录组数据证实了在盐应激下补充Se的反应中,塑性APX的上调调节.

结论:

作为APX的强有力的酶调节剂.
低度的Se有效地减轻了狐尾小米中NaCl诱导的盐度压力.
补充Se增强了植物的抗氧化剂防御系统,改善了盐分耐受性.

关键词:

无生物应激应激是一种非生物应激.缓解减轻的作用过氧化是一种过氧化.在体分析中进行体分析.SiAPX (塞塔利亚意大利酸盐过氧化酶)

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