用超声波协助制备蛋白质-多烯酸合物及其结构和功能特性
Shizhang Yan1, Qi Wang1, Jiaye Yu1
1College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Ultrasonics sonochemistry
|October 14, 2023
概括
超声波增强大豆蛋白分离物 (SPI) 和表甲基酸盐 (EGCG) 结合,优化功能性质,如乳化和抗氧化活性,特别是通过处理.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 大豆蛋白分离物 (SPI) 是一种有价值的蛋白质来源,具有功能修饰的潜力.
- (-) - 乙甲基酸盐 (EGCG) 是一种强大的多,具有抗氧化和促进健康的特性.
- 将SPI与EGCG结合起来可以增强两个组件的功能和应用范围.
研究的目的:
- 研究超声波对制备SPI-EGCG结合物的常规方法的协同效应.
- 评估EGCG接种对SPI结构和功能特性的影响.
- 确定生产多功能SPI-EGCG复合物的最佳条件.
主要方法:
- 使用超声波辅助处理,自由基调解和酶方法制备SPI-EGCG合物.
- 使用反应组分析,光谱分析 (例如FTIR,CD) 和表面疏水性测量分析结构变化的分析.
- 功能性质的评估,包括乳化,抗氧化活性和α-葡萄糖酶抑制.
- 分子对接模拟以了解相互作用机制.
主要成果:
- 超声波在和自由基方法中提高了聚烯接种效率,同时在酶方法中降低了效率.
- 植入EGCG形成了与SPI的C-N和C-S键,降低了α-螺旋体含量,增加了分子灵活性,并诱导了键/疏水相互作用.
- 超声波辅助处理产生了SPI-EGCG结合物,具有最佳的乳化,抗氧化特性和α-葡萄糖酶抑制活性.
结论:
- 超声波是一种有效的协同工具,用于制备具有增强功能的SPI-EGCG结合物.
- 超声波辅助处理为创建多功能SPI-EGCG复合体提供了最佳方法.
- 这项研究通过有针对性的功能化扩大了SPI高价值利用的潜力.
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