抗蛋白的结冰部位不可逆转地与细胞表面结合,以实现其低温保护功能
Yue Yang1, Akari Yamauchi2, Sakae Tsuda3
1Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, 277-8561, Japan.
Biochemical and biophysical research communications
|October 14, 2023
概括
抗蛋白 (AFPs) 通过不可逆转地与细胞结合,保护细胞免受寒冷损伤. 这种结合对于低温细胞保护 (HCP) 至关重要,与它们的结结冰机制不同.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 低温生物学 低温生物学
背景情况:
- 抗蛋白 (AFP) 通过抑制冰晶生长来保护生物体免受寒冷的影响.
- 对于AFP的低温细胞保护 (HCP) 功能的机制尚未完全理解.
- AFPs附着于细胞表面,可能提供对寒冷引起的损伤的保护.
研究的目的:
- 研究来自鱼类的AFPs (I,II和III类型) 低温细胞保护 (HCP) 功能的机制.
- 为了确定AFP结冰活动和HCP功能之间的关系.
- 阐明细胞吸附在AFP介导的冷保护中的作用.
主要方法:
- 使用人类T-淋巴细胞淋巴瘤细胞在4°C保存24小时,对I,II和III类AFP的HCP功能进行评估.
- 在不同AFP度下测量细胞存活率.
- 在III型AFP的结冰部位 (IBS) 引入点突变,以评估其对HCP和结冰活动的影响.
- 通过用新鲜介质取代含有AFP的溶液来研究AFP细胞结合的可逆性.
主要成果:
- 所有测试的AFP都以度依赖的方式提高了细胞存活率.
- 与I型和II型AFP相比,III型AFP的HCP效率较低.
- 突变III型IBS的AFP显著增加了HCP活动,这表明IBS参与了细胞吸附.
- 一种抗活性降低的突变体表现出增强的HCP活性,表明了结冰和细胞保护的独特机制.
- I型和II型AFP在4°C时与细胞结合不可逆转,密集吸附的分子对HCP至关重要.
结论:
- 在低温下,AFP的低温细胞保护 (HCP) 功能通过不可逆转的细胞吸附作用.
- 由AFP介导的细胞保护机制与其结冰功能不同.
- 细胞表面吸附,而不是冰结合抑制,是AFP对细胞冷保护作用的主要驱动因素.
更多相关视频
09:43Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
Published on: May 5, 2017
8.7K
09:32LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
Published on: February 4, 2013
20.7K
相关概念视频
Introduction to Plant Diversity
44.8K
From Water to Land
44.8K
Drug Binding to Blood Components
155
When drugs enter systemic circulation, they interact with various components of the blood, including proteins such as human serum albumin (HSA), α1-acid glycoprotein (AAG), lipoproteins, globulins, and red blood cells (RBCs).
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are...
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are...
155
Responses to Heat and Cold Stress
13.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.5K
Antibody Actions
1.1K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
1.1K
Ligand Binding Sites
12.9K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.9K
Antibody Structure
60.2K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
60.2K
