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用不同的矩阵编制的校准标准对使用LC-MS/MS进行胆酸量化准确性的影响研究

Jinni Yang¹, Jiachun Lin¹, Anhui Wang²

¹China Pharmaceutical University Nanjing Drum Tower Hospital, Nanjing 210009, China; Key Laboratory of Drug Quality Control & Pharmacovigilance (China Pharmaceutical University), Ministry of Educational, Nanjing 210009, China.

Journal of pharmaceutical and biomedical analysis

|October 14, 2023

概括

使用质谱法对代谢物的绝对量化正在取得进展. 使用真实矩阵校准标准可以提高胆酸测量的准确性,为生物样本提供可靠的策略.

科学领域:

分析化学分析化学
生物化学生物化学
代谢学代谢学代谢学

背景情况:

基于质谱的代谢学旨在实现实验室间数据可比性的绝对量化.
由于缺乏用于校准标准的无分析物矩阵,对内源分子的绝对量化具有挑战性.
胆汁酸 (BAs) 是疾病进展中的关键生物标志物.

研究的目的:

为了评估内源胆汁酸在不同的矩阵中准备的校准曲线的定量准确性.
引入和验证一个两步校准策略,以改善胆酸量化.

主要方法:

使用整洁溶剂 (NSCC),木炭剥离矩阵 (SMCC) 和真实矩阵 (AMCC) 准备了校准曲线.
使用在真实矩阵中准备的质量控制样本 (QC) 验证了定量准确性.
在不同的校准方法中比较精度和置信区间.

主要成果:

与NSCC和SMCC相比,真实矩阵校准标准 (AMCC) 显著减少了置信区间和精度偏差.
当测量度超过背景水平的20%时,AMCCs的好处最为明显.
拟议的两步校准策略证明了内源BA量化可靠性.

结论:

真正的矩阵校准对于准确的内源代谢物如胆酸的绝对量化至关重要.
开发的两步校准策略为生物样本中的胆酸量化提供了强大而可靠的方法.
这种方法提高了不同实验室的代谢学数据的可比性和可靠性.

关键词:

准确度准确度准确度准确度准确度真正的矩阵.胆汁酸胆汁酸是一种校准标准校准标准校准标准替代矩阵是一个替代矩阵.

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