针对传染性脑炎的疫苗接种会引起可检测的NS1 IgG抗体反应
Rahel Ackermann-Gäumann1, Arthur Brêchet2, Jan Smetana3
1Microbiologie, ADMED Analyses et Diagnostics Médicaux, La Chaux-de-Fonds, Switzerland; Swiss National Reference Center for Tick-Transmitted Diseases, Switzerland.
Journal of virological methods
|October 14, 2023
概括
人类接种感染性脑炎病毒 (TBEV) 疫苗可以诱导非结构蛋白1 (NS1) 特定抗体. 虽然这些疫苗诱导的抗体的水平通常很低,但要将它们与自然感染标记物区分开来,需要仔细的切断界限定义.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 疫苗学 疫苗学 疫苗学
背景情况:
- 性脑炎病毒 (TBEV) 包膜蛋白的抗体介导疫苗的保护.
- 非结构蛋白1 (NS1) 特定抗体通常是自然TBEV感染的标志物.
- TBEV疫苗可能含有NS1痕迹,可能诱导NS1特异性抗体.
研究的目的:
- 调查TBEV疫苗是否会在人类中诱导NS1特异性抗体.
- 为了比较接种疫苗和未接种疫苗的个体中的NS1特异性抗体水平.
- 评估NS1特异性抗体在区分疫苗接种与自然TBEV感染中的有用性.
主要方法:
- 健康的军队成员 (n=898) 和疑似TBE患者 (n=71) 提供了血液样本和疫苗接种/感染史.
- 一个内部开发的ELISA选了TBEV NS1特异性IgG抗体的样本.
- 抗体量化使用了百分比阳性;定性评估基于接种疫苗的个体数据定义了截止值.
主要成果:
- 接种疫苗的人与未接种疫苗的人相比,NS1特异性IgG抗体标位显著更高.
- 接种疫苗的参与者中有更高的百分比对NS1特异性IgG抗体呈阳性.
- 虽然标位低于TBE患者,但基于NS1抗体的疫苗接种与感染的区别并不总是清楚.
结论:
- 人类TBEV疫苗接种可以诱导可检测的NS1特异性IgG抗体.
- 疫苗接种后NS1特异性抗体的存在使其作为自然感染的唯一标记物的使用变得复杂.
- 精确的切断值对于区分疫苗诱导和感染诱导的NS1特异性抗体反应至关重要.
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