牛乳酸通过CD36/Fyn/FAK/mTORC1轴在牛乳腺上皮细胞中促进脂质合成

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Xiaoru Yang¹, Xinyue Lu², Liping Wang¹

¹State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010021, China.

International journal of biological macromolecules

|October 14, 2023

概括

牛乳酸通过CD36和Fyn/FAK/mTORC1信号传递促进牛乳细胞中的脂质合成. 这一途径调节脂肪的产生,提供了对食脂肪酸利用的见解.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
动物科学动物科学

背景情况:

酸 (SA) 是一种和长链脂肪酸 (LCFA),对于奶牛的哺乳非常重要.
饮食中的SA储存为三糖醇 (TAG).
通过FAK/mTORC1信号传导,CD36 (脂肪酸转位酶) 在SA诱导的脂质合成中的作用尚不清楚.

研究的目的:

研究牛乳腺上皮细胞 (BMECs) 中CD36介导的酸诱导的脂质合成机制.
阐明FAK/mTORC1信号通路在这个过程中的参与.

主要方法:

在BMEC中使用了SA丰富补充剂.
使用了抗CD36抗体和CD36抑制剂SSO.
过度表达的CD36.
使用TAE226/拉帕米辛抑制了FAK/mTORC1.
进行了共免疫沉和酵母双杂交试验.

主要成果:

在BMEC中,SA增强了脂质合成和FAK/mTORC1通路活性.
抗CD36和SSO损害了SA诱导的脂质合成和信号;CD36过度表达增强了它.
抑制FAK/mTORC1降低了SA诱导的TAG合成和脂原基因表达.
确定了一个信号轴:CD36与Fyn相互作用,Fyn与FAK结合;FAK与TSC2相互作用,与mTORC1.1结合.

结论:

甜酸通过涉及Fyn/FAK/mTORC1信号的CD36依赖途径促进BMEC中的脂质生成.
这项研究揭示了乳腺细胞中LCFA诱导的脂质合成的新型分子机制.

关键词:

牛乳腺上皮细胞牛乳腺上皮细胞 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 这是假的,假的,假的.菲恩 (Fyn Fyn) 是一个很好的地方.牛油酸是什么牛油酸是什么牛油酸是什么在 mTORC1 的情况下,mTORC1 是

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