丝虫的糖氨基甘与Bombyx mori核多重体病毒结合,并促进其进入
Weiwei Zhou1, Jindie Hong1, Jinying Han1
1School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
International journal of biological macromolecules
|October 14, 2023
概括
博姆比克斯·莫里核多面体病毒 (BmNPV) 使用甘氨酸甘氨酸 (GAG),特别是肝素硫酸 (HS) 和胆酸硫酸 (CS),作为感染的附着因子. 针对这些GAG可以抑制BmNPV病毒的进入和复制.
科学领域:
- 病毒学 病毒学
- 葡萄糖生物学 葡萄糖生物学
- 分子生物学分子生物学
背景情况:
- 病毒感染始于病毒颗粒与宿主细胞表面受体或附着因子之间的相互作用.
- 在真核细胞表面上丰富的甘氨酸经常成为病毒的目标.
- 作为一个重要的农业害虫,Bombyx mori核多重体病毒 (BmNPV) 的感染机制仍然不完全理解.
研究的目的:
- 调查糖氨基甘氨酸 (GAG) 修饰的作用,特别是肝硫酸 (HS) 和胆固醇硫酸 (CS),在BmNPV感染中.
- 为了确定潜在的宿主受体,促进BmNPV的进入.
主要方法:
- 酶去除细胞表面的HS和CS,以评估它们对BmNPV感染的影响.
- 在体外结合测试使用外源HS和CS与BmNPV病毒.
- 在BmNPV注射后对敏感和耐性丝虫菌株的GAG生物合成酶表达的分析.
主要成果:
- 酶去除HS和CS显著抑制了BmNPV感染和复制.
- 外源性HS和CS直接与BmNPV病毒结合,中和病毒感染性.
- BmNPV感染导致敏感丝虫的GAG生物合成酶表达升调,但在耐药菌株的表达下调.
结论:
- 肝硫酸盐 (HS) 和胆固醇硫酸盐 (CS) 作为BmNPV入口的关键附着因子.
- BmNPV似乎操纵宿主细胞机械,以增强GAG生物合成,使其受益.
- 准HS和CS是抑制BmNPV感染的潜在策略.
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