细胞图形分析揭示了sirtuin的不同演变,这可能源于III类酶
Yujiao Yang1, Siwei Zou2, Kezhu Cai3
1CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; University of Chinese Academy of Sciences, Beijing, China.
The Journal of biological chemistry
|October 14, 2023
概括
赛尔图因,NAD+依赖性脱糖酶,在整个生命中表现出多样化的活动. 这项研究揭示了它们的进化分歧,起源于III类的Sirtuins,为它们的调节和进化提供了新的见解.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 赛尔图因是NAD+依赖的脱糖酶,对诸如衰老和基因调节等细胞过程至关重要.
- 哺乳动物的Sirtuins表现出除了脱乙化之外的多种脱乙酶活动.
- 了解不同生命领域的Sirtuin进化和活动是有限的.
研究的目的:
- 系统地分析古生物,细菌和真核生物中的Sirtuin活动和Zymographs.
- 为了研究Sirtuins的分子进化和进化起源.
- 提供关于Sirtuins细胞特异性调节和进化分歧的见解.
主要方法:
- 对于Sirtuin活动概况的HPLC测定.
- 基于7-amino-4-methylcoumarin的化法用于酶活性检测.
- 遗传学分析以追踪进化关系.
主要成果:
- 在全球范围内,Sirtuin脱酶活动被分为三个主要类型.
- 第三类sirtuins,特别是来自细菌和古生物,显示无差异的zymographs.
- 在III类Sirtuins中,在paralog和ortholog水平上观察到酶活性的初始差异化,这表明该类的演变不同.
结论:
- 赛尔图因进化很可能起源于III类赛尔图因,显示出不同的进化路径.
- 这项研究提供了对各种物种的Sirtuin发射图的全面概述.
- 介绍了关于Sirtuins细胞调节和分子进化的新见解.
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