帕奇马兰对氧化应激和甲引起的DNA损伤的影响
Zhijun Zhang1, Yuan Yang1, Changjun Hu2
1College of Public Health and Laboratory Medicine, Hunan University of Medicine, 492 Jinxi South Road, Huaihua, Hunan, 418000, People's Republic of China.
Scientific reports
|October 14, 2023
概括
帕奇马兰有效地抑制甲诱导的氧化应激和小鼠的遗传损伤. 这项研究表明,帕奇马兰可以增加抗氧化酶活性 (超氧化脱酶和谷氨减少酶),并以剂量依赖的方式降低脂质过氧化 (马隆迪) 和DNA损伤标志物.
科学领域:
- 药理学 药理学是指药理学的学科.
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 甲是一种已知的氧化应激和遗传损伤诱导剂.
- 帕奇马兰是一种具有潜在药理作用的化合物,需要进行调查.
- 了解天然化合物对毒性侮辱的保护机制对于开发治疗策略至关重要.
研究的目的:
- 为了研究帕奇马兰对甲诱导的氧化应激的抑制作用.
- 评估帕奇马兰对甲诱导的遗传损伤的保护作用.
- 为了确定帕奇马兰的保护作用的剂量效应关系.
主要方法:
- 成年雄性昆明小鼠被随机分配到四个实验组,进行不同的干预.
- 小鼠接受治疗一周,随后进行血清分析.
- 酶相关免疫吸收试验 (ELISA) 用于量化血清中超氧化脱酶 (SOD),谷氨缩酶 (GR),氨酸 (MDA),DNA-蛋白质交叉连接 (DPC),8-氧化氨酸 (8-OHDG) 和DNA添加剂的水平.
主要成果:
- 帕奇马兰的使用显著改变了SOD,GR和MDA的血清水平 (P <0.01).
- SOD和GR活动增加,而MDA水平随着帕奇马兰剂量的增加而下降,表明氧化应激减少.
- 帕奇马兰治疗剂量取决于DPC,8-OHDG和DNA添加物的水平降低 (DPC和DNA添加物的P < 0.01;8-OHDG的P < 0.05),这意味着对遗传损伤的保护.
结论:
- 帕奇马兰对甲诱导的氧化应激有显著的抑制作用.
- 帕奇马兰在减轻甲诱导的DNA损伤方面发挥着保护作用.
- 对于帕奇马兰对甲毒性的保护作用,存在明确的剂量效应关系.
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