土壤细菌通过感知竞争对手的细胞内IVA型分泌系统效应器来操纵抗真菌武器
Bingxin Wang1, Zeyu Zhang1, Fugui Xu1
1College of Plant Protection (State Key Laboratory of Biological interactions and Crop Health; Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, 210095, P.R. China.
The ISME journal
|October 14, 2023
概括
土壤细菌使用接触依赖的武器来触发抗真菌抗生素的生产. 这种相互作用保护植物免受真菌病原体的影响,揭示了新的微生物通信策略.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 生物化学 生物化学
背景情况:
- 土壤有益细菌利用依赖接触的系统,如IVA型分泌系统 (T4ASS),以抑制病原体.
- 这些T4ASS武器在生物型微生物相互作用中的作用在很大程度上仍未被探索.
研究的目的:
- 调查T4ASS在土壤细菌之间的物种间相互作用中的参与.
- 阐明一种土壤细菌可以诱导另一种土壤细菌产生抗真菌化合物的机制.
主要方法:
- 在甲板上共同培养Pseudomonas蛋白质和Lysobacter酶原体,以模拟细胞与细胞的接触.
- 负责诱导抗真菌生产的T4ASS效应蛋白的识别和特征.
- 对基因表达和蛋白质与蛋白质相互作用的分析,以了解调节机制.
主要成果:
- 莱索巴克特酶原体通过T4ASS触发了Pseudomonas蛋白质中的2,4-DAPG抗生素的产生.
- 来自L. enzymogenes的T4ASS效应蛋白LtaE被转移到P.蛋白质中,在那里它激活了2,4-DAPG生物合成.
- LtaE与抑制剂PhlF直接相互作用,缓解抑制并刺激抗生素的产生,从而保护大豆幼苗免受Rhizoctonia solani的侵害.
结论:
- 描述了一种由T4ASS调解的微生物物种间和王国间相互作用的新机制.
- 这一发现扩大了已知的土壤微生物相互作用和沟通策略的多样性.
- 这项研究提供了一个独特的细菌T4ASS介导植物保护抗真菌化合物的诱导的例子.
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