在肉样本中分析生物氨基的新方法:使用2-chloro-3-nitropyridine的微波辅助衍生
Aneta Jastrzębska1, Anna Kmieciak1, Zuzanna Gralak1
1Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 7 Gagarin Str., 87-100 Toruń, Poland.
Food chemistry
|October 15, 2023
概括
使用2-chloro-3-nitropyridine的新方法有效地合成生物氨基衍生物用于肉质分析. 尸体成为肉类腐败的关键指标,无论肉类是什么类型.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 有机化学 有机化学
背景情况:
- 肉类中的生物氨基酸表明微生物污染,并影响质量.
- 准确的生物胺检测方法对于确保肉类安全至关重要.
- 目前的方法需要优化,以提高效率和可靠性.
研究的目的:
- 引入2-chloro-3-nitropyridine作为微波辅助生物氨基衍生物化的新试剂.
- 开发和验证一种HPLC方法,用于量化肉类中的生物氨基.
- 评估生物氨基胺作为肉质指标和储存期间变化的有用性.
主要方法:
- 使用2-chloro-3-nitropyridine的生物氨基衍生物的微波辅助合成.
- 使用NMR (1H, 13C) 和高分辨率质谱学对合成衍生物的表征.
- 使用HPLC测量肉样本 (肉,火腿,胸) 中生物氨基酸的数量.
主要成果:
- 在合成生物氨基衍生物中获得的高纯度和产量.
- 成功地应用衍生和HPLC方法来分析真肉样本.
- 鉴定尸体作为存储期间 (24h和72h) 肉类变化的主要指标.
结论:
- 2-chloro-3-nitropyridine是生物氨基衍生物化的有效试剂.
- 开发的HPLC方法为肉类质量评估提供了可靠的工具.
- 尸体水平可以作为监测不同类型肉类腐败的强有力的标志物.
更多相关视频
08:14Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
Published on: December 9, 2022
3.8K
08:00Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Published on: November 4, 2021
2.3K
相关概念视频
01:202° Amines to N-Nitrosamines: Reaction with NaNO2
4.3K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
4.3K
01:19Mass Spectrometry of Amines
4.2K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule: a molecule with an odd number of nitrogen atoms produces a parent ion with an odd molecular weight. The remaining fragments have an even mass.
Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit...
Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit...
4.2K
01:19Amines to Amides: Acylation of Amines
2.5K
Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
2.5K
01:38Preparation of Amines: Reductive Amination of Aldehydes and Ketones
2.9K
Carbonyl compounds and primary amines undergo reductive amination first to produce imines, followed by secondary amines in the same reaction mixture, using selective reducing agents like sodium cyanoborohydride or sodium triacetoxyborohydride. Reductive amination produces different degrees of substitution of amines depending on the starting amine substrate.
2.9K