超低温高压处理增强了诺罗病毒替代物的无活化
Christina A M DeWitt1, Kevin A Nelson1, Hyung Joo Kim1
1Coastal Oregon Marine Experiment Station, Seafood Research and Education Center, Oregon State University, Astoria, OR, USA.
International journal of food microbiology
|October 15, 2023
概括
超低温高压处理 (HPP) 显著增强了像MNV和TV这样的诺罗病毒替代品的失活,特别是当应用于解样本时. 这种非热方法在食品安全应用中提供了改善的病原体控制.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 食品安全 食品安全
背景情况:
- 高压处理 (HPP) 是一种非热方法来使病原体无活化.
- 人类诺罗病毒在0°C以上的温度下需要>400 MPa才能显著无活化.
- 鼠类诺基病毒 (MNV) 和图兰病毒 (TV) 被用作替代物来研究诺基病毒失活.
研究的目的:
- 为了评估MNV和TV对超低温HPP的敏感性.
- 为了比较HPP在解和冷样本上的失活效率.
- 评估超冷HPP在等食物矩阵中对病原体控制的潜力.
主要方法:
- MNV和TV样品平衡到+1.5°C (解) 或-40°C (冷).
- 用超低温HPP (-40°C室内液体) 在200,250或300MPa下5分钟加压样本.
- 在解和冷样本中确定了病毒日志的减少,并评估了中的TV无活化.
主要成果:
- 对于解样本,MNV在200MPa时实现了>4.4日志减少,在250-300MPa时实现了>6.1日志减少. 在测试压力中,TV实现了2.3-5日志减少.
- 对于冷样品,MNV实现了2.3-4.2日志减少,TV在测试压力中实现了0.81-2.3日志减少.
- 在测试的超低温HPP压力下,在中证明了TV无活化.
结论:
- 超冷HPP增强了与较高温度相比,诺罗病毒替代物的无活化.
- 应用于解样本的HPP显示出对MNV和TV的优越无活化效果.
- 超低温HPP显示出作为有效的食品安全干预措施的希望,特别是在像这样的产品中禁用病毒.
相关概念视频
Physical Methods for Controlling Microbial Growth: Temperature
32
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
32
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
36
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
36
Methods of Sterilization II: Chemical Methods
6.5K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
6.5K
Methods of Sterilization I: Physical Methods
20.1K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
20.1K
Factors Influencing Microbial Growth: Temperature
22
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
22


