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在糖尿病视网膜病变中视网膜微血管内皮功能障碍中的eIF4A3介导的circEHMT1调节

Yuan Wang¹, Yongxin Zhang¹, Yunhao Qu²

¹Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China; Shenzhen Eye Institute, Shenzhen 518040, China.

Microvascular research

|October 15, 2023

概括

高葡萄糖降低了糖尿病视网膜病变 (DR) 中的circEHMT1和eIF4A3. eIF4A3调节circEHMT1,改善内皮细胞功能和改善大鼠的DR,提供潜在的治疗点.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

糖尿病视网膜病变 (DR) 与圆形RNAs (circRNAs) 有关.
circEHMT1维持内皮细胞屏障的功能.
调节circEHMT1的机制及其在DR病原发生中的作用需要研究.

研究的目的:

研究调节DR中circEHMT1表达的机制.
确定circEHMT1及其调节器eIF4A3在DR病原发生中的作用.
探索DR的治疗潜力.

主要方法:

暴露于视网膜微血管内皮细胞的高葡萄糖 (HG).
评估了管形成,细胞间接口蛋白 (ZO-1,克劳丁-5,奥克卢丁),circEHMT1和eIF4A3.3.
采用了一种链毒素 (STZ) 诱导的DR大鼠模型.

主要成果:

HG 降低了circEHMT1,eIF4A3,ZO-1,Claudin-5 和 Occludin 的水平.
eIF4A3 调节了 circEHMT1 的表达.
过度表达eIF4A3或circEHMT1改善了内皮细胞损伤和管形成.
在体内,eIF4A3过度表达恢复了circEHMT1并改善了DR相关的血管变化.

结论:

eIF4A3调节circEHMT1的表达,影响微血管内皮细胞损伤和管形成.
了解eIF4A3-circEHMT1相互作用可能会为DR揭示新的治疗点.

关键词:

这是循环RNARNA.内皮细胞内皮细胞的内皮细胞.内皮细胞损伤,糖尿病视网膜病变高葡萄糖的情况.一个圆圈EHMT1 在 eIF4A3A3 中.

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