枣种子废弃物衍生纳米催化剂及其用于生产水解酶,发酵糖和生物的应用
Ali H Bahkali1, Asad Syed1, Abdallah M Elgorban2
1Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
Bioresource technology
|October 15, 2023
概括
这项研究开发了一种枣种子纳米催化剂,以促进从枣纸废物中提升纤维化酶的产生. 纳米催化剂增强了酶活性和稳定性,使得高效的葡萄糖和生物生产成为可能.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 可再生能源可再生能源是可再生能源.
背景情况:
- 纤维素生物燃料的生产受到昂贵的纤维溶解酶的阻碍.
- 来自枣种子的纳米催化剂为降低酶生产成本提供了潜在的解决方案.
- 对可持续生物燃料的倡议来说,对日纸废物的价值化至关重要.
研究的目的:
- 用于从枣子种子中制备纳米催化剂.
- 通过固态发酵评估纳米催化剂对纤维溶解酶生产的影响.
- 评估酶衍生葡萄糖和生物生产的效率.
主要方法:
- 从枣子种子制成的纳米催化剂制剂.
- 使用细菌共同培养的日纸废物的固态发酵.
- 酶活性和稳定性测试 (过纸活性,热和pH稳定性).
- 预处理的枣种子的水解,用于生产葡萄糖.
- 黑暗发酵用于生物生成.
主要成果:
- 在优化条件下,用2毫克的纳米催化剂产生了30 IU/gds的过纸活性.
- 在纳米催化剂的存在下,细胞质表现出增强的热稳定性 (50°C,pH 7 10小时).
- 32.31 g/L的葡萄糖是通过水解从预处理的枣种子中生产的.
- 在24小时内通过共同培养的暗发酵产生了1788毫升/升的累积H2.
结论:
- 枣种子纳米催化剂显著增强细胞分解酶的产生和稳定性.
- 这种综合方法可以有效地将日废弃物转化为葡萄糖和生物等有价值的产品.
- 该研究提出了生物燃料生产和废物回收利用的成本有效策略.
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