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通过人类中性粒细胞来调节皮肤纤维细胞

Nattarika Niwetbowornchai^1,2, Thanawat Chaisirirat³, Sira Sriswasdi^3,4

¹Center of Excellence in Translational Research in Inflammation and Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Scientific reports

|October 15, 2023

概括

人类中性粒细胞 (HNP) 促进纤维细胞的增殖和原蛋白的产生,表明治疗潜力. 这些发现突出了HNP的存在.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
皮肤病学皮肤病学

背景情况:

人类中性粒细胞 (HNP) 已知会影响细胞生长和激活.
目前正在探索它们在促进组织修复和再生方面的潜在临床应用.
了解HNP对皮肤纤维细胞的影响对于治疗开发至关重要.

研究的目的:

研究HNP对人类皮肤纤维细胞增殖和激活的影响.
识别受HNP影响的差异表达基因和相关的细胞内通路.
评估HNP在伤口愈合和组织工程中的潜力.

主要方法:

人体皮肤纤维细胞被分离并培养在2D和3D系统中.
用HNP处理细胞,并评估增殖 (Ki-67) 和激活 (COL1A1) 标记物.
用RNA测序 (RNA-seq) 来分析差异性基因表达和途径分析 (KEGG,基因本体学).

主要成果:

在没有诱导细胞毒性的情况下,HNP显著增加了纤维细胞增殖.
在2D和3D培养中,HNP1增强了COL1A1表达和I型原蛋白的产生.
RNA-seq揭示了不同的基因表达模式,高调基因与增殖/激活相关,低调基因与脂质代谢/炎症相关.

结论:

HNP刺激人类皮肤纤维细胞的增殖和激活,这表明潜在的临床益处.
特别地,HNP1促进了原蛋白的合成,这表明它在伤口愈合和组织再生中发挥了作用.
HNP调节细胞通路,减少炎症和脂质代谢,同时增强与生长相关的过程.

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