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在Flavobacterium columnare中,基因组证明了遗传多样性和功能进化

Rui Han^1,2, Yuhao Hong¹, Ruilong Xu¹

¹University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, China.

Frontiers in microbiology

|October 16, 2023

概括

在Flavobacterium columnare的全基因组测序中,发现了显著的遗传变异和水平基因转移. 结果表明F.columnare可能代表多个不同的物种,需要进行分类学重新评估.

科学领域:

微生物学微生物学
基因组学就是基因组学.
水产养殖病理学水产养殖病理学

背景情况:

在淡水鱼类中,Flavobacterium columnare会引起columnaris病,导致大量的水产养殖损失.
了解F. columnare的遗传组成对于疾病管理和预防至关重要.

研究的目的:

使用全基因组测序来描述Flavobacterium columnare的基因组.
研究F. columnare种群中的遗传多样性,可塑性和进化机制.
根据基因组数据评估F. columnare的分类地位.

主要方法:

F. columnare 的全基因组测序是 AH-01 和 GX-01 的分离物.
鉴定基因组岛屿,预兆区域和CRISPR/Cas系统.
使用16S rRNA,家政基因和核心基因组比较的家族遗传学分析.
包括平均核酸标识 (ANI) 在内的比较基因组分析.

主要成果:

对F. columnare分离物AH-01和GX-01的基因组进行了测序,揭示了基因含量和基因组结构的差异.
在两种基因组中都发现了基因变异,横向基因转移,基因组岛屿和传染区域的证据.
遗传学和ANI分析将55F.columnare分离成四个不同的分类,其群体间的分歧表明是单独的物种.
核心基因组仅占总基因组的25%左右,这表明基因组具有相当大的可塑性.

结论:

细菌 (Flavobacterium columnare) 呈现出由基因变异和水平转移驱动的显著遗传多样性.
基因组数据强烈表明,F. columnare是多个不同的物种的复合体,而不是单一的实体.
根据所提供的基因组证据,建议对F. columnare进行修订的分类学分类.

关键词:

在Flavobacterium的柱子里.cgMLST 的意思进行比较的基因组学.全基因组分析 (GWA)一个基因的基因结构.

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