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干扰素兰巴达3和4基因SNP的关联及其表达与COVID-19疾病严重程度:一个跨部分研究

Warisha Zahid¹, Nida Farooqui², Nida Zahid³

¹Department of Community Health Sciences, Aga Khan University, Karachi, Pakistan.

Infection and drug resistance

|October 16, 2023

概括

干扰素羔羊4 (IFNL4) 基因单核酸多态 (SNP) 和患者年龄与COVID-19的严重程度有关. 这些因素,以及IFNL3/4基因表达,可以作为更好的SARS-CoV-2感染管理的生物标志物.

科学领域:

免疫遗传学免疫遗传学
病毒学病毒学
基因组学就是基因组学.

背景情况:

干扰素兰巴达3和4 (IFNL3/4) 基因表达和单核酸多态 (SNP) 显示与全球COVID-19结果的不同关联.
人口特定的遗传因素可能会影响COVID-19疾病的严重程度.

研究的目的:

在巴基斯坦患者中调查IFNL3/4SNP (rs12979860和rs368234815) 及其鼻表达与COVID-19严重程度的关联.

主要方法:

对来自轻度和重度COVID-19病例的117个鼻样本进行分析.
针对病毒载量和IFNL3/4mRNA表达的定量PCR (qPCR).
分别为IFNL3和IFNL4SNP进行四臂PCR和RFLP-PCR.
使用曼·惠特尼U测试,奇方测试和多变量考克斯回归的统计分析.

主要成果:

IFNL4基因SNP (rs368234815) 显示了轻度和重度COVID-19组之间的统计学上显著差异 (p=0.001).
IFNL3的mRNA表达是3.48倍低,IFNL4的mRNA表达是3.48倍高的严重病例相比轻度病例.
多变量分析确定IFNL4 SNP和年龄与COVID-19严重程度有显著关联.

结论:

IFNL4基因SNP和患者年龄与研究的巴基斯坦人口中的COVID-19严重程度有关.
IFNL3/4 mRNA表达和IFNL4 SNP可能作为预测SARS-CoV-2感染严重性的生物标志物.
识别这些生物标志物可能有助于改善COVID-19患者管理策略.

关键词:

COVID-19 的严重程度.在 IFNL3 IFNL3 国际金融基金会 (IFNL4)干扰素兰巴达是一种干扰素.单个核酸的多形态.

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