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使用整个化细胞和无细胞提取物在2-二的存在下化活性:批量和SBR测定

J E Pérez-Alfaro¹, A Villaseca¹, Raúl Gaytán¹

¹Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Iztapalapa, CP 09310 Mexico City, México.

|October 16, 2023

概括

高度的氨使得化细菌能够同时降解2-二 (2-CP) 和氨. 这项研究揭示了2-CP对氨运输和化酶的影响,这对生物修复策略至关重要.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
废水处理废水处理

背景情况:

化细菌对于从废水中去除氨是必不可少的.
化有机化合物如2-二 (2-CP) 可以抑制化.
了解氨度,2-CP和化活性之间的相互作用对于有效治疗至关重要.

研究的目的:

为了研究不同度对2 - CP在一个化联盟的同时降解的影响.
阐明2-CP抑制化及其与化酶相互作用的机制.
评估测序批量反应器 (SBR) 对于同时化和2-CP去除的有效性.

主要方法:

使用批量和SBR培养的整个化细胞进行动力分析.
用化无细胞提取物进行实验,以区分运输和酶作用.
不同的氨 (NH4+-N) 度 (100-500 mg/L) 和一个恒定的2-CP度 (5 mg/L).

主要成果:

100毫克NH4+-N/L与2-CP.没有发生化.
在SBR中观察到200-500毫克NH4+-N/L的同时氧化,氧化和2-CP消耗.
氨基单氧酶酶参与了2-CP的消耗,而2-CP对酸盐氧化物的抑制作用在较高的氨基水平下减弱.
无细胞提取物显示2-CP消耗,但保留了抑制作用,表明对运输和酶的影响.

结论:

的高度 (200-500 mg/L) 促进了化协会的同时化和2-CP降解.
2-CP 抑制了氨运输和化酶活性.
SBR在同时去除氨和2-CP方面是有效的,这表明了增强生物修复策略的潜力.

关键词:

氨氧化的氨氧化过程运输氨的运输方式没有细胞的提取物.抑制抑制的抑制作用膜膜是一种膜.

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