阐明双语PNA生物聚合物的序列组装关系
Hector Argueta-Gonzalez1, Colin S Swenson1, Kornelia J Skowron2
1Department of Chemistry, Emory University, Atlanta, Georgia 30322, United States.
ACS omega
|October 16, 2023
概括
研究人员创建了双语核酸 (PNA) 生物聚合物,将核酸和蛋白质语言结合起来. 修改氨基酸特性允许微调自组装和拆卸,以实现潜在的治疗应用.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 合成生物学 合成生物学
背景情况:
- 像核酸和蛋白质这样的生物聚合物有不同的信息存储和功能"语言".
- 目前的生物聚合物仅限于编码单一类型的语言.
- 核酸 (PNA) 支架可以将核酸和氨基酸残留物整合到单个骨干中.
研究的目的:
- 研究不同氨基酸特性如何影响双语PNA生物聚合物的自我组装和拆卸.
- 阐明氨基酸序列,两性和细胞形成之间的关系.
- 评估结构修改对核酸识别和刺激响应分解的影响.
主要方法:
- 一系列具有多种氨基酸残留 (不同长度,电荷,疏水性,间距) 的双语PNA序列的合成.
- 使用技术来确定粒大小和临界粒度的自我组装性质的表征.
- 对每个PNA变种的核酸识别和拆卸机制的评估.
主要成果:
- 两胞性双语PNAs通过氨基酸残留的指导自我组装成状结构.
- 水友性的负电荷或重的疏水性侧链促进了组装成大小相似的微粒,负电荷增加了临界微粒度.
- 切断PNA序列导致较小的自组装结构,同时保持识别和拆卸能力.
- 所有变体都表现出序列特异性识别和刺激响应性拆解.
结论:
- 两性双语生物聚合物的氨基酸和核酸序列可以精确地设计,以控制自组装和拆卸.
- 这些可调节的特性对于封装和治疗货物的有针对性传递等领域的应用至关重要.
- 双语PNA技术为开发具有定制功能的先进生物材料提供了一个多功能平台.
更多相关视频
09:04Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
9.6K
09:34Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
Published on: February 6, 2020
7.3K
相关概念视频
03:36Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
02:41Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
02:28Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K