对芳香氨基酸的肠道微生物代谢物的质谱分析
Narumol Jariyasopit1,2, Sakda Khoomrung1,2,3
1Siriraj Center of Research Excellence in Metabolomics and Systems Biology (SiCORE-MSB), Faculty of Medicine Siriraj Hospital Mahidol University, Bangkok 10700, Thailand.
Computational and structural biotechnology journal
|October 16, 2023
概括
研究人员审查了用于分析肠道微生物群衍生芳香氨基酸 (AAA) 代谢物的质谱法. 本摘要有助于理解它们在疾病中的作用和开发生物标志物.
科学领域:
- 微生物组研究 微生物组研究
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
背景情况:
- 来自肠道微生物群的小分子对于了解人类肠道微生物组的功能至关重要.
- 芳香氨基酸 (AAA) 的微生物代谢产物与各种疾病有关,包括代谢障碍,病,炎症性肠病,糖尿病和癌症.
- 全球代谢物分析是发现这些重要的微生物AAA代谢物在生物样本中的关键.
研究的目的:
- 总结最近 (过去五年) 基于质谱 (MS) 的方法分析肠道微生物群衍生的AAA代谢物的进展.
- 提供对这些代谢物的样本准备,方法验证,分析性能和统计相关性分析的见解.
- 强调准确识别和定量AAA代谢物对生物标志物开发的重要性.
主要方法:
- 复杂质谱法 (MS) 技术的审查,在代谢物分析中以它们的选择性和灵敏性而受到重视.
- 讨论各种生物矩阵中识别和量化AAA代谢物的方法.
- 检查样本准备,方法验证和分析性能考虑.
主要成果:
- 基于MS的技术对于微生物AAA代谢物的选择性和敏感的识别和量化至关重要.
- 标准化方法和验证对于比较研究结果和确定代谢物生物标志物值至关重要.
- 最近的发展重点是改进基于MS的方法来分析这些关键的肠道微生物化合物.
结论:
- 基于质谱的方法显著提高了肠道微生物群衍生的AAA代谢物的分析.
- 准确的量化和验证对于将这些代谢物与疾病状态联系起来,以及开发可靠的生物标志物至关重要.
- 预计持续的方法开发将进一步阐明这些代谢物在人类健康和疾病中的功能作用.
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