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转录基因和遗传方法显示,低光诱导的疾病易感性与番茄细胞氧化应激有关

Qian Luo¹, Jiao Wang¹, Ping Wang¹

¹Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

Horticulture research

|October 16, 2023

概括

低亮度增加了番茄病的易感性,通过损害抗氧化防御和提高氧化应激. 这影响了作物安全,强调了在低光环境中需要新的保护策略.

科学领域:

植物病理学植物病理学
植物生理学植物生理学
分子生物学分子生物学

背景情况:

低光强度对全球作物安全构成重大威胁,导致大量的收获损失.
低光影响植物防御反应的确切机制在很大程度上仍未被描述.
了解这些机制对于制定有效的作物保护战略至关重要.

研究的目的:

为了研究低光条件对番茄易受*Pseudomonas syringae* pv的影响. *西红* DC3000 (Pst DC3000). *西红* DC3000 (Pst DC3000). *西红* DC3000 (Pst DC3000). *西红* DC3000 (Pst DC3000). *西红* DC3000 (Pst DC3000). *西红* DC3000 (Pst DC3000). *西红* DC3000 (Pst DC3000).
阐明氧化应激和抗氧化剂反应在低光诱导疾病易感性中的作用.
探索潜在的干预措施,以减轻低光下的疾病.

主要方法:

RNA测序 (RNA-seq) 用于分析在不同光线条件下的基因表达变化.
测量反应性氧物种 (ROS) 积累和蛋白质氧化.
酶活性测定抗氧化酶,包括阿斯科巴特过氧化酶2 (APX2).
编辑CRISPR-Cas9基因以创建apx2*突变体,并评估外源性酸盐 (AsA) 治疗.

主要成果:

低光加剧Pst DC3000诱导的番茄植物中的ROS积累和蛋白质氧化.
在病原体接种后,抗氧化剂防御基因和酶活动,包括APX2,在弱光下被抑制.
降低的酸盐与脱酸盐 (AsA/DHA) 的比率在弱光下降,表明氧化应激增加.
*apx2*突变体在低光下对Pst DC3000表现出更高的敏感性,这在AsA治疗下得到改善.

结论:

番茄的低光诱导疾病易感性与抗氧化能力受损和细胞氧化应激增加有关.
抗氧化途径的破坏,特别是涉及APX2,在弱光下会加剧疾病.
研究结果表明,加强抗氧化剂防御可能是改善低光环境中的作物弹性的一种可行策略.

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