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Updated: Jul 13, 2025

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Quantitative 31P NMR Analysis of Lignins and Tannins
Published on: August 2, 2021
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作为一种"通用"的参考,TBS-pyrrole用于量化NMR的植物提取物中的素和结构多样化的天然产品
Ana L García-García1,2, Dácil Hernández1, Álvaro Santana-Mayor3
1Grupo de Síntesis de Fármacos y Compuestos Bioactivos, Instituto de Productos Naturales y Agrobiología del Consejo Superior de Investigaciones Científicas (CSIC), La Laguna, Spain.
Frontiers in plant science
|October 16, 2023
概括
量化天然产品,如明素可能是昂贵的. 使用通用参考化合物的新核磁共振 (NMR) 方法为大规模分析提供了快速,准确和低成本的替代方案.
科学领域:
- 自然产品化学 自然产品化学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 有价值的自然产品的商业生产依赖于具有可变产量的植物来源.
- 精确量化各种自然产品是必不可少的,但标准方法 (HPLC,UHPLC) 是昂贵和复杂的.
- 对于自然产品而言,需要一种可扩展,具有成本效益的量化方法.
研究的目的:
- 开发和验证一种使用核磁共振 (NMR) 来量化各种自然产品的新,简单和快速的方法.
- 为自然产品量化建立一个"通用"的参考标准.
- 为了证明该方法的有效性,用阿尔特米西宁和一个模型植物提取物.
主要方法:
- 开发一种使用TBS-pyrrole作为新型"通用"参考标准的核磁共振 (NMR) 量化协议.
- 使用新型NMR方法量化素,并与标准UHPLC-MS程序进行比较.
- 应用NMR协议来量化各种生物活性化合物 (烯,类固醇,类化合物等). 在一个模型植物提取物中.
主要成果:
- 这种新型的NMR方法提供了与UHPLC-MS.相比的精确量化结果.
- TBS-pyrrole参考化合物没有干扰分析剂信号,只需要少量样本.
- 该方法在量化复杂植物提取物中的多种生物活性化合物方面表现出卓越的准确性.
- 该NMR协议在操作上简单,允许高通量样本处理.
结论:
- 使用通用参考化合物 (TBS-pyrrole) 的基于NMR的新型量化方法为传统方法提供了简单,快速和准确的替代方案.
- 这种方法大大降低了成本,提高了大规模自然产品量化效率.
- 该方法具有多功能性,适用于广泛的生物活性天然产品,支持商业生产和研究.
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