静脉注射番茄莱克来评估功能性血管系统
Carey L Watson1,2, Maxime M Mahe1, Michael A Helmrath1,2
1Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, USA.
Bio-protocol
|October 16, 2023
概括
人类多能干细胞创建功能小肠组织模型. 来自宿主小鼠的血管化对于肠道成熟至关重要,可视化使用西红讲蛋白.
科学领域:
- 干细胞生物学 干细胞生物学
- 胃肠病学 胃肠病学
- 血管生物学 血管生物学
背景情况:
- 多能干细胞使得体外和体内器官模型的创建成为可能.
- 这些模型有助于研究器官功能,生理学,胚胎学和病理学.
- 一个实验室开发的人类小肠模型在体内成熟,类似于成人肠道.
研究的目的:
- 为了研究移植的人类小肠组织的血管化.
- 了解宿主来源的血液供应在肠道组织成熟中的作用.
- 开发一种可视化工程肠道组织内的血管系统的方法.
主要方法:
- 人类多能干细胞被用来生成小肠模型.
- 工程化肠道组织被移植到小鼠宿主体中,用于体内成熟.
- 用光素标记的Lycopersicon esculentum (番茄) 莱克被静脉注射以标记内皮.
主要成果:
- 移植的人体肠道组织成熟成一个像成年人的结构.
- 组织成熟度取决于宿主提供足够的血液供应的发展.
- 番茄莱克成功地可视化了移植肠道组织内的血管网络.
结论:
- 人类多能干细胞衍生的肠组织可以在体内成熟.
- 主体血管化对于成熟的肠组织的发展至关重要.
- 番茄培养素是研究人工肠道移植的血管系统的有效工具.
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