相关实验视频

Updated: Jul 13, 2025

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

老化CAR-T细胞对与年龄相关的代谢功能障碍的预防性和长期有效性

Corina Amor¹, Inés Fernández-Maestre^2,3, Saria Chowdhury¹

¹Cold Spring Harbor Laboratory. Cold Spring Harbor, NY, USA.

Research square

|October 16, 2023

概括

衰老细胞驱动衰老的衰退. 使用 CAR T 细胞向 uPAR 的新疗法有效清除这些细胞,通过单剂量改善老年模型的代谢健康和身体健康.

科学领域:

细胞衰老细胞衰老
免疫治疗是一种免疫疗法.
衰老的研究研究.

背景情况:

衰老细胞随着年龄的增长而积累,导致组织功能障碍和与年龄有关的疾病.
消除衰老细胞可以逆转与年龄相关的病理,但目前的衰老药物有局限性.
化学抗原受体 (CAR) T细胞为衰老细胞清除提供了有针对性的方法.

研究的目的:

为了研究在生理衰老过程中PAR阳性衰老细胞的积累.
评估老化CAR T细胞针对UPAR的疗效,以改善与年龄相关的代谢功能障碍.
为了确定这些CAR T细胞的单次低剂量管理的长期治疗和预防作用.

主要方法:

开发的CAR T细胞准衰老细胞标记物UPAR.
向老年小鼠和代谢综合征模型中使用抗uPAR CAR T 细胞.
评估代谢功能,耐葡萄糖性和运动能力.

主要成果:

在生理衰老过程中确认了UPAR阳性衰老细胞的积累.
证明老化CAR T细胞安全地准并消除这些细胞.
在治疗后,葡萄糖耐受性和运动能力显著改善.
从单次低剂量治疗中观察到的长期治疗和预防作用.

结论:

老龄化CAR T细胞代表了一种有前途的基于细胞的疗法,用于与年龄相关的衰退.
单次低剂量给药可以实现长期的治疗和预防效果.
这种方法改善了代谢功能障碍,并提高了衰老中的身体健康.

更多相关视频

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

相关概念视频

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Autophagy

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...