对乳腺癌中与阿诺基斯相关的基因的预后分析
Jingyu Cao1, Xinyi Ma2, Guijuan Zhang3
1Department of Oncology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
PeerJ
|October 16, 2023
概括
与阿诺基斯相关的基因 (ARG) 在乳腺癌 (BRCA) 进展和转移中至关重要. 确定关键的ARG可以帮助预测患者的生存率,并改善BRCA的治疗策略.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 乳腺癌 (BRCA) 是全球癌症死亡的主要原因.
- 阿诺基斯的耐药性是BRCA转移和治疗失败的关键驱动因素.
- 在BRCA中,无基因相关基因 (ARG) 的预后价值尚不清楚.
研究的目的:
- 建立基于ARG的BRCA预后模型.
- 确定与患者存活率和瘤进展相关的ARG.
- 探索ARG在BRCA转移和信号通路中的作用.
主要方法:
- 使用了癌症基因组图谱 (TCGA) 和基因表达总汇 (GEO) 数据库.
- 应用最小绝对收缩和选择操作员 (LASSO) 分析来构建预后模型.
- 进行了基因本体学 (GO),基因和基因组的京都百科全书 (KEGG) 和通路分析.
- 使用卡普兰-梅尔曲线,接收器运行特征 (ROC) 曲线和定量聚合酶连锁反应 (qPCR) 验证的关键ARG.
主要成果:
- 开发了一个预后模型,识别出具有显著生存差异的不同低风险和高风险群体.
- 确定了11种与瘤进展和转移相关的差异表达的ARG.
- 在高危人群中发现了Notch和Wnt信号通路的过度表达.
- 验证了五个关键的ARG (CD24,KRT15,MIA,NDRG1,TP63) 作为3年生存的重要预测因素.
结论:
- 与阿诺基斯相关的基因在BRCA进展和转移中发挥着关键作用.
- 开发的预后模型和确定的ARG可以作为BRCA预后的有价值指标.
- 对这些ARG的进一步研究可能会导致乳腺癌的新疗法策略.
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