Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

5.6K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.6K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
The Ras Gene02:38

The Ras Gene

6.2K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.2K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

4.0K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.6K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Quantitative proteomics identifies surfactant-resistant alpha-synuclein in cerebral cortex of Parkinsonism-dementia complex of Guam but not Alzheimer's disease or progressive supranuclear palsy.

The American journal of pathology·2007
Same author

Different supramolecular assemblies in two 1:1 proton-transfer compounds of sulfobenzoic acids with aromatic amines.

Acta crystallographica. Section C, Crystal structure communications·2007
Same author

Identification of proteins involved in microglial endocytosis of alpha-synuclein.

Journal of proteome research·2007
Same author

Biomarkers for Alzheimer's disease.

Expert review of neurotherapeutics·2007
Same author

[Role of sympathetic nerve activity and arterial endothelial function in pathogenesis of hypertension in patients with obstructive sleep apnea-hypopnea syndrome].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2007
Same author

Subsequently enhanced CPP to morphine following chronic but not acute footshock stress associated with corticosterone mechanism in rats.

The International journal of neuroscience·2007

相关实验视频

Updated: Jul 13, 2025

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:39

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

164

过度活跃的KRAS/MAPK信号破坏了正常的淋巴血管结构和功能.

Lorenzo M Fernandes1, Jeffrey Tresemer1, Jing Zhang2

  • 1Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center, Dallas, TX, United States.

Frontiers in cell and developmental biology
|October 16, 2023
PubMed
概括

激活KRAS突变会通过破坏淋巴内皮细胞 (LEC) 发育,导致复杂的淋巴异常 (CLA). 用特拉美丁尼布抑制MEK1/2显示出通过恢复正常的淋巴血管形成来治疗CLA的希望.

关键词:
戈勒姆 - 斯托夫病是什么?克拉斯 (Kras) 是一个国家.复杂的淋巴异常 淋巴异常淋巴血管生成 (Lymphangiogenesis) 是一种导致淋巴血管发生的过程.淋巴细胞形的错误形成特拉美丁尼布 (trametinib) 是一种药物.

更多相关视频

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

14.4K
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.0K

相关实验视频

Last Updated: Jul 13, 2025

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:39

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

164
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

14.4K
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.0K

科学领域:

  • 血管生物学 血管生物学
  • 发育生物学是发展生物学.
  • 分子遗传学 分子遗传学

背景情况:

  • 复杂淋巴异常 (CLA) 是一种罕见的疾病,源于淋巴血管发育不良.
  • 在KRAS的体质激活突变与CLA病变发生有关.
  • 关联KRAS突变与CLAs的确切机制尚不清楚.

研究的目的:

  • 阐明KRAS信号在淋巴内皮细胞 (LEC) 功能和淋巴发育中的作用.
  • 研究向KRAS驱动的CLA中的MAPK通路的治疗潜力.

主要方法:

  • 在胚胎淋巴内皮细胞 (LEC) 和人类初级LEC中KRASG12D的表达.
  • 评估LEC形态,增殖,迁移和基因表达的评估.
  • 在体外和体内使用特拉美丁尼布进行MEK1/2的药理抑制.
  • 在胚胎模型中分析淋巴血管形成和增生.

主要成果:

  • 在LEC中KRASG12D的表达会损害淋巴静脉形成,扩大淋巴血管,并改变LEC的行为 (螺旋,增殖,迁移).
  • KRASG12D增加了AKT和ERK1/2的酸化,并降低了与成熟相关的基因的调节.
  • 特拉美替尼治疗逆转了KRASG12D诱导的变化,减少了ERK1/2酸化,并提高了成熟基因的调节.
  • 在胚胎模型中,MEK1/2抑制抑制了KRASG12D诱导的淋巴细胞增生.

结论:

  • 严格调节KRAS/MAPK信号传递对于胚胎发生过程中淋巴血管正常发育至关重要.
  • 抑制MEK1/2代表了通过激活KRAS突变引起的CLA的潜在治疗策略.