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对一些用于检测狂犬病病毒的技术进行比较评估

Dalia N Hegazy¹, Ebtesam N Hosseny¹, Amal S M Abo-Senna¹

¹Faculty of Science, Al-Azhar University Girl's Branch, Nasr, Egypt.

Open veterinary journal

|October 16, 2023

概括

准确的狂犬病病毒检测对于疾病控制至关重要. 传统的PCR和直接光抗体方法 (DFAT) 提供了高准确度,而快速抗原套件提供了更快,虽然不那么敏感,狂犬病诊断的结果.

科学领域:

兽医病毒学兽医病毒学
诊断微生物学诊断微生物学
公共卫生公共卫生

背景情况:

狂犬病是一种致命的脑膜炎,由Rhabdoviridae家族的神经型病毒引起,属Lysavirus.
快速和准确的狂犬病病毒 (RABV) 诊断对于疾病控制和减轻公共卫生风险至关重要.

研究的目的:

评估用于RABV检测的各种诊断技术.
确定最简单,最快速,最准确的测试,以在不同标本中识别RABV.

主要方法:

瑞士小鼠和BHK21细胞系感染了具有挑战性的RABV菌株.
使用:BHK21细胞系感染,直接光抗体技术 (DFAT),乳聚合试验 (LAT),直接与酶相关的免疫吸收试验 (ELISA),狂犬病抗原检测工具包ELISA和常规聚合酶链反应 (PCR) 检测RABV.

主要成果:

小鼠和细胞系接种显示出90%-100%的阳性,但需要5-7天.
快速抗原套件在3-5分钟内检测到狂犬病抗原 (55.5%-60%的阳性).
在1-1.5小时内,DFAT实现了90%-100%的阳性.
在3-5分钟内,LAT显示了88.8%的阳性.
传统的PCR在2天内产生了100%的阳性.
直接ELISA在1天内显示了88.8%的阳性.

结论:

小鼠接种,细胞培养,DFAT和PCR是最准确的RABV检测方法 (90%-100%的阳性).
拉特和ELISA显示了88.8%的阳性.
狂犬病抗原ELISA套件 (RAK) 的阳性率较低 (55.5%-60%),但提供了快速的结果.
测试的准确性,灵敏度,特异性和周转时间是狂犬病诊断的关键因素.

关键词:

光抗体技术是一种光抗体技术.乳凝结法乳凝结法接种小鼠疫苗的小鼠.这是一个PCRPCR.狂犬病病毒的病毒.

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