相关实验视频

Updated: Jul 13, 2025

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

来自Rehmannia glutinosa的两种多糖:隔离,结构特征和低血糖活动

Huien Chen^1,2,3, Xinyu Liu^1,2,3, Meixia Xie^1,2,3

¹Guangdong Pharmaceutical University Guangzhou 510006 China gdpuwsm@126.com xmh360@163.com.

|October 16, 2023

概括

来自Rehmannia glutinosa (RG) 的两种新型同聚糖体通过抑制关键的消化酶显示出显著的低血糖效应. 一种多糖也刺激GLP-1分泌,这表明它有可能成为2型糖尿病管理的功能性食物.

科学领域:

药理学和天然产品化学
生物化学和分子生物学

背景情况:

雷曼尼亚 (Rehmannia glutinosa,简称RG) 是一种具有药用和食品用途的中国传统草药.
RG多糖因其低血糖作用而闻名,但RG同聚糖的活性尚不清楚.

研究的目的:

为了隔离和表征RG同聚糖糖类.
为了研究这些孤立的多糖的低血糖活性和作用机制.

主要方法:

使用染色学技术分离和净化两个RG同聚糖 (RGP70-1-1和RGP70-1-2).
结构分析包括单糖组成,链接分析 (FT-IR,HPLC,GC-MS,NMR),刚果红色试验和SEM.
在体外酶抑制试验 (α-葡萄糖酶和α-氨基酶).
在STC-1细胞上进行体外细胞研究,以评估GLP-1分泌和相关蛋白质表达 (PI3K,p-AKT).

主要成果:

两个同聚糖,RGP70-1-1 (4.9 kDa) 和RGP70-1-2 (2.8 kDa),成功地被分离和表征.
这两种多糖都由葡萄糖,曼诺糖,阿拉比诺糖和银河糖组成,具有复杂的链接模式.
RGP70-1-1和RGP70-1-2对α-葡萄糖酶和α-氨基酶表现出抑制作用.
在STC-1细胞中,RGP70-1-1显著刺激了类似葡萄糖-1 (GLP-1) 的分泌,与PI3K/p-AKT通路激活相关.

结论:

这项研究阐明了两种新型RG同聚糖的结构和低血糖潜力.
这些化合物显示出作为碳水化合物消化抑制剂和GLP-1分泌剂的前景.
RGP70-1-1和RGP70-1-2代表了管理2型糖尿病的潜在功能性食品成分.

更多相关视频

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Published on: June 25, 2018

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

相关概念视频

Chemistry of Carbohydrates

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are...

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...