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通过牛乳酪蛋白调节HeLa细胞增殖和细胞亡

Xingfu Shu^1,2, Jinxian Su^1,2, Yu Zhao^1,2

¹Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, China.

Cell biochemistry and function

|October 16, 2023

概括

牛乳酸 (bLF) 通过诱导亡和自来抑制宫癌细胞的生长. 这项研究澄清了bLF.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
在瘤学瘤学.

背景情况:

宫癌仍然是女性在全球的重大健康问题.
乳酸 (LF) 具有潜在的抗瘤特性.
了解bLF对宫癌细胞的机制至关重要.

研究的目的:

为了研究牛乳酸 (bLF) 对子宫癌HeLa细胞增殖和亡的影响.
阐明bLF对HeLa细胞的作用的潜在分子机制.

主要方法:

用CCK-8,Trypan蓝色染色和殖民地形成试验来评估细胞增殖.
通过 Hoechst 33258 染色,AO/EB 染色和西式斑点评价了亡和自.
在BLF诱导的亡中自的作用使用抑制剂3-MA.被检查.

主要成果:

在剂量和时间上,bLF显著抑制了HeLa细胞的增殖.
bLF通过对p53,Bax和Cleaved-caspase-3进行上调和对Bcl-2进行下调来诱导亡.
bLF激活了自,由增加的LC3B-II和Beclin I证明,并减少了p62.
自抑制逆转了BLF诱导的亡,表明BLF通过自促进了亡.

结论:

bLF有效地抑制了宫癌的HeLa细胞增殖.
bLF在HeLa细胞中诱导了亡和自.
bLF的抗癌作用是通过激活自的调解,后者随后触发了亡.

关键词:

灭症 (apoptosis) 是一种死亡的过程.自自是自的过程.牛乳酪蛋白是牛乳酪蛋白中的一种.宫癌:子宫癌是一种癌症.它们的扩散和扩散.

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