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组织处理方法的全面比较,用于高质量的MALDI在重建的人类表皮中的脂质成像

Maureen Feucherolles¹, William Le¹, Jérôme Bour¹

¹Luxembourg Institute of Science and Technology (LIST), Molecular and Thermal Analysis, Materials Research and Technology, L-4422 Belvaux, Luxembourg.

Journal of the American Society for Mass Spectrometry

|October 16, 2023

概括

优化的样本准备提高了矩阵辅助激光脱/离子化质谱成像 (MALDI MSI) 用于重建人体表皮 (RHE) 模型中的脂质分析. 这种方法可以更好地评估皮肤成分变化的皮肤化品.

科学领域:

生物医学成像学生物医学成像学
分析化学是一种分析化学.
皮肤病学皮肤病学

背景情况:

矩阵辅助激光脱/电离质谱成像 (MALDI MSI) 对于皮肤分析至关重要,使分子检测和定位成为可能.
重建人体表皮 (RHE) 模型为研究化学效应提供了一个可行的,伦理的替代人体皮肤.
关于将MALDI MSI应用于RHE模型的研究有限,这凸显了对优化协议的需求.

研究的目的:

调查样品制备技术对MALDI MSI质量的影响,用于SkinEthic RHE模型中的脂质分析.
为了确定基质,样品厚度,洗和矩阵应用的最佳条件,以增强脂质成像.

主要方法:

使用大气压MALDI (AP-MALDI) 与高分辨率质谱仪 (5微米像素大小) 相结合.
评估了各种样品制备参数:涂层金属基板 (例如,APTES涂层的不钢),6微米的组织厚度和水洗.
使用LipostarMSI平台分析检测到的质量和注释的离子.

主要成果:

在HCCA矩阵喷涂之前,APTES涂层金属基板,6微米RHE段和水性洗的组合产生了优异的MALDI MSI结果.
这种优化的协议显著增加了信号强度和脂质检测到的m/z值的数量.
发现矩阵的再结晶是不必要的,并且可能有害.

结论:

精细的AP-MALDI MSI方法,包括特定的样本准备步骤,增强RHE模型中的脂质分析.
这种改进的工作流可以更准确地评估皮肤成分的变化由皮肤美容产品诱导.
该研究提供了一个强大的协议,用于推进皮肤研究,使用RHE模型和先进的质谱成像.

关键词:

AP-MALDI 马尔代夫马尔代夫马尔代夫脂质脂质脂质是一种质谱仪成像成像质谱仪成像成像重建的人类表皮.样品的准备样品的准备皮肤皮肤皮肤

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