在多发性硬化症中,microRNA-155和炎症媒介之间的关系
Rania Elsayed1, Salwa Fayez1, Laila Ahmed Rashed1
1Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Journal of biochemical and molecular toxicology
|October 16, 2023
概括
多发性硬化症 (MS) 中的微RNA-155 (miRNA-155) 表达与炎症标志物相关,这表明它在调节免疫细胞反应中的作用. 这一发现为MS患者的神经炎症机制提供了潜在的见解.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
背景情况:
- 多发性硬化症 (MS) 是一种影响中枢神经系统的慢性自身免疫性疾病,其特征是神经炎症,脱髓化和轴突损伤.
- 微RNA-155 (miRNA-155) 被认为是巨细胞和微质细胞中关键的促炎调节剂,影响其功能极化.
研究的目的:
- 在患有复发性复发性硬化症 (RRMS) 的患者中研究miRNA-155的血水平.
- 评估miRNA-155水平与RRMS中关键的炎症和抗炎介质之间的关联.
主要方法:
- 这项研究涉及60名多发性硬化症患者和30名健康对照.
- 实时定量聚合酶连锁反应 (RT-qPCR) 用于测量miRNA-155,iNOS和SMAD2.
- 酶相关免疫吸收试验 (ELISA) 用于量化TNF-α,IFN-ɣ,TGF-β和IL-10水平.
主要成果:
- 在MS患者和对照人群之间,没有发现miRNA-155,SMAD2或iNOS表达的显著差异.
- 在MS患者中观察到TNF-α,IFN-ɣ和TGF-β水平的统计显著增加.
- IL-10水平在两组之间没有显著差异.
- 在miRNA-155和TNF-α,IFN-ɣ和iNOS之间发现了显著的正相关性.
- 在miRNA-155和IL-10,TGF-β和SMAD2.2之间观察到逆相关性.
结论:
- 在MS患者中的血miRNA-155表达与促炎性标记物 (TNF-α,IFN-ɣ,iNOS) 有正相关,与抗炎性标记物 (IL-10,TGF-β,SMAD2) 有负相关.
- 这些发现表明,miRNA-155可能在调节多发性硬化中的巨细胞和微质细胞两极分化方面发挥作用,可能是通过影响这些介质的分泌.
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