高压鼻腔刺激的抗病毒作用
V Frari1, A Micera, F Bonifacio
1Research Unit of Otorhinolaryngology, Campus Biomedico University, Rome, Italy. f.bonifacio@policlinicocampus.it.
European review for medical and pharmacological sciences
|October 16, 2023
概括
高压无菌盐溶液 (HPpSIS) 治疗激活了鼻子的先天免疫力,并可能提供抗病毒益处. 这种疗法可提高Toll类受体和eosinophil衍生的神经毒素的调节,这表明它具有保护作用.
科学领域:
- 免疫学 免疫学 免疫学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 分子生物学分子生物学
背景情况:
- 之前的研究表明,在高压施用无菌盐酸同位素溶液 (HPpSIS) 后,鼻神经生长因子 (NGF) 增加.
- 在HPpSIS后,鼻腔粘膜的先天免疫反应和细胞因子概况需要进一步的表征,特别是关于抗病毒特性.
研究的目的:
- 在HPpSIS之后,描述鼻腔粘膜的先天免疫反应和细胞因子特征.
- 调查潜在的抗病毒益处和NGF在HPpSIS治疗中的作用.
主要方法:
- 在HPpSIS之前和之后,从20名患者的鼻腔样本进行了形态,生化 (ELISA) 和分子 (RT-PCR) 分析.
- 量化了托尔类受体 (TLR3,TLR7,TLR9),互白蛋白 (IL18,IL13,IL12),乙氨基基基标记物 (EDN,ECP),干扰素- (γIFN),酶和血清素的转录.
主要成果:
- 通过HPpSIS治疗,乙氨基基细胞,巨细胞以及对ECP/RNase3和EDN的免疫活性增加.
- 观察到血清激素和EDN水平升高,以及TLR3,TLR7,TLR9和ECP/RNase3转录的显著上调.
- 在组胺,物质P,ICAM1,IL18,IL15,IL12,IL6,IL13,γIFN或三酶转录中没有发现显著的变化.
结论:
- HPpSIS治疗似乎通过托尔类受体转录激活了鼻腔先天性免疫监测.
- 观察到的乙氨基基基衍生神经毒素 (EDN) 的上调表明一种潜在的抗病毒反应.
- 需要进一步的研究来确认局部释放的NGF是否有助于这些观察到的效应.
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