对用于核酸分离的聚合物zwitterionic水友性单体的综合评估
Liang Lai1, Mengyun Zhang1, Chusheng Liu1,2
1Institute of Pharmaceutical Analysis, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou, 510632, China.
概括
一种新型的zwitterionic水友单体,poly(SPP-co-MBA),提供核酸 (NT) 和其衍生物的快速和稳定的分离. 这种方法显示出极好的HILIC-MS兼容性,在NT分析中有效.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 准确分离核酸 (NTs) 和其衍生物对于理解生物过程至关重要.
- 现有的方法在稳定性和有效性方面面临着全面NT分析的挑战.
研究的目的:
- 为了评估一个zwitterionic水友性单体的分离能力,聚N,N-二甲基-N-3-甲基胺) -N-3-硫) 氨基贝他因-co-N,N'-甲基二甲基胺) (聚SPP-co-MBA)).
- 为了评估其选择性,化学稳定性和水友相互作用液体色谱学与质谱学 (HILIC-MS) 兼容性,用于NT分析.
主要方法:
- 综合评估了多单体 (SPP-co-MBA) 的性能.
- 优化分离条件,包括缓冲器pH值,度,有机溶剂含量和列温度.
- 在性条件下 (pH10) 通过4000次连续注射评估化学稳定性.
主要成果:
- 聚-SPP-co-MBA) 单体表现出优异的化学稳定性,保持时间的相对标准偏差很低 (0.16-1.05%).
- 在7分钟内实现了四种核酸三酸盐,五种二酸盐和五种单酸盐的基线分离.
- 核化物及其相应酸盐的混合物仅在3分钟内就被分离了.
- 九个NT的满意的峰值形状和高响应证实了良好的HILIC-MS兼容性.
结论:
- 聚-SPP-co-MBA) 单体表现出强大的机械稳定性和出色的HILIC-MS兼容性.
- 这种zwitterionic水友单体为分析核酸及其衍生物提供了一种有前途且高效的技术.
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