Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

231
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
231
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

378
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
378

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Exploring Bahia's coffee diversity: NMR spectroscopy and chemometrics unveil regional variations.

Food chemistry·2025
Same author

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens.

Metabolites·2024
Same author

Liquid Chromatography-Mass Spectrometry for Clinical Metabolomics: An Overview.

Advances in experimental medicine and biology·2021
Same author

Analytical Platforms for Mass Spectrometry-Based Metabolomics of Polar and Ionizable Metabolites.

Advances in experimental medicine and biology·2021
Same author

Anti-Inflammatory and Antinociceptive Properties of Kielmeyerone A, a Chromenone Isolated from the Roots of <i>Kielmeyera reticulata</i>.

Journal of natural products·2021
Same author

Untargeted Metabolomics Insights into Newborns with Congenital Zika Infection.

Pathogens (Basel, Switzerland)·2021

相关实验视频

Updated: Jul 13, 2025

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

33.7K

微生物代谢学中的样品制备:进步和挑战

Heiter V M Boness1, Hanna C de Sá1, Emile K P Dos Santos1

  • 1Department of Analytical Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, BA, Brazil.

Advances in experimental medicine and biology
|October 16, 2023
PubMed
概括

微生物代谢学揭示了微生物生理学. 适当的样本准备对于可靠的结果至关重要,这会影响该领域的数据质量.

关键词:
细胞培养 细胞培养细胞外代谢组细胞内代谢组是细胞内代谢组.代谢火的消火方式提取代谢物的提取代谢物.微生物的代谢物.样品的准备 样品的准备

更多相关视频

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
07:56

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Published on: September 3, 2012

15.4K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.6K

相关实验视频

Last Updated: Jul 13, 2025

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

33.7K
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
07:56

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Published on: September 3, 2012

15.4K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.6K

科学领域:

  • 微生物学 微生物学
  • 代谢学 代谢学 代谢学
  • 生物化学 生物化学

背景情况:

  • 微生物代谢学对于理解微生物生理学至关重要.
  • 代谢物度和生物体的可变性需要仔细的样品准备.
  • 样本准备直接影响代谢数据的质量和可靠性.

研究的目的:

  • 为了提供对微生物代谢学样本制备的全面概述.
  • 突出代谢工作流程中关键的预分析因素.
  • 讨论获取细胞内和细胞外代谢物的方法.

主要方法:

  • 关于微生物代谢学样本准备工作流程的概述.
  • 讨论分析前的因素:种植,收获,代谢火和提取.
  • 介绍先进的自动化样品准备系统.

主要成果:

  • 样品准备程序取决于微生物类型,代谢学方法 (有针对性/无针对性) 和分析平台.
  • 提出了有效的方法来分离细胞内和细胞外代谢物.
  • 先进的自动化技术在样本准备中提供了更高的效率.

结论:

  • 优化样品制备对于准确的微生物代谢学是必不可少的.
  • 选择的方法会影响代谢物分析的范围和可靠性.
  • 自动化系统代表了微生物代谢学样本准备的未来方向.