3D声波稀疏激活局部化显微镜与相变对比剂的相变对比剂
Kai Riemer1, Qingyuan Tan, Sophie Morse
1From the Department of Bioengineering, Imperial College London, London, United Kingdom (K.R., Q.T., S.M., M.T., J.Y., J.Z., B.W., L.T., M.L., P.D.W., M.-X.T.); NDORMS, University of Oxford, Oxford, United Kingdom (L.B., Q.W., E.S.); and Department of Physics, Imperial College London, London, United Kingdom (C.D.).
Investigative radiology
|October 16, 2023
概括
这项研究介绍了用于体内微血管成像的3D声波稀激活局部化显微镜 (AWSALM). 这种新的技术提供了增强的分辨率和透度,用于用相变对比剂可视化血液流动.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 超声波技术 超声波技术 超声波技术
- 微血管研究 微血管研究
背景情况:
- 目前的超声波技术在解决微血管结构方面存在局限性.
- 需要先进的成像方法,以高分辨率可视化复杂的血管网络.
研究的目的:
- 为了展示3D声波稀激活局部化显微镜 (AWSALM) 在体内微血管成像.
- 为了利用相变对比剂 (PCCA) 进行增强的超声波局部化显微镜.
主要方法:
- 使用可声激活PCCA开发和应用3D AWSALM.
- 在微流幻影,子脏和老鼠大脑中通过完整的头骨评估了该技术.
- 使用多重超声波阵列和自定义算法进行图像重建.
主要成果:
- 实现了64μm的空间分辨率,比传统方法有了显著的改进.
- 证明了PCCA激活的时空控制,用于按需对比.
- 在小鼠中展示了跨脑成像和微血管学中改善了信号局部化.
结论:
- 3D AWSALM提供体积超声波超分辨率微血管图像在体内.
- 该技术提供了时空选择性和增强的微血管透.
- AWSALM代表了高分辨率体内血管成像技术的重大进步.
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