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切割压力和微RNAs用于更好的转移性癌症管理

Siluveru KrishnaPriya¹, Pallavi S Nair¹, Prerna Bhalla¹

¹Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences Building-1, Indian Institute of Technology Madras, Chennai, India.

Biotechnology progress

|October 16, 2023

概括

剪切应激可以通过影响微RNA (miRNA) 和活性氧物种 (ROS) 来禁用转移的癌细胞. 这项研究表明,特定的miRNAs和ROS水平与结肠癌细胞失活相关,提供治疗点.

科学领域:

生物物理学的生物物理.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

转移,癌细胞的扩散,导致90%的癌症死亡,这是一个重大的临床挑战.
剪切应力是一种所有迁移癌细胞所经历的物理力.
活性氧物种 (ROS) 和microRNAs (miRNAs) 涉及癌细胞失活.

研究的目的:

为了研究剪切应力对结肠癌细胞中的miRNAs和ROS的影响.
探索针对癌症转移管理的剪切诱导分子变化的潜力.

主要方法:

HCT116结肠癌细胞被培养在一个圆板装置中,以施加受控的剪切应力.
细胞生长特征和特定的细胞内ROS (si-ROS) 水平在不同的剪切速率下被测量.
分析了特定miRNAs (hsa-miR-335-5p,hsa-miR-34a-5p,hsa-miR-26b-5p) 和ROS相关的mRNA (PMAIP1) 的表达水平.

主要成果:

在1.57s-1及以上的剪切速率下,HCT116细胞生长受到抑制.
剪切应激调节了特定的miRNA表达:hsa-miR-335-5p被上调,而hsa-miR-34a-5p被下调.
特定的miRNA水平与细胞内基水平负相关,PMAIP1mRNA在剪切下显示差异性表达.

结论:

切割压力影响关键的分子介质 (miRNAs,ROS) 参与癌细胞失活.
已确定的miRNAs (hsa-miR-335-5p,hsa-miR-34a-5p) 和可能与ROS相关的mRNAs代表了结肠癌转移的有希望的治疗标.

关键词:

HCT116结肠癌细胞中的结肠癌细胞这是一种基基.转移转移转移转移这是一个微型RNA.有反应性物种的反应性物种.剪切压力的压力.这是一种超氧化物.

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