基于aptamer的特殊识别,以高保真度的光激活,双模式光和色度检测雌激醇
Zihua Guo1, Bin Yang1, Jian Zhu1
1Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 410005, PR China.
Food chemistry
|October 16, 2023
概括
本研究提出了一种双模式测定,用于快速且可靠地检测食品中的雌醇残留物. 这种创新方法提供了敏感和选择性的检测,对于监测危害健康的内分泌干扰化学物质至关重要.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 食品中的雌醇残留物对健康构成风险,需要准确的检测方法.
- 多模式测定为复杂样本分析提供了更高的可靠性和抗干扰性.
- 现有的检测方法可能缺乏常规监测所需的速度,灵敏度或可靠性.
研究的目的:
- 开发一种快速,可靠和灵敏的双模式检测试剂来检测雌激醇.
- 为了提高检测准确度,利用光和色度信号.
- 为了验证该方法在真实食品样本中的选择性和适用性.
主要方法:
- 开发了一种结合光和色度检测的双模式检测方法.
- 这种方法利用提奥夫拉T与雌激醇胺体结合,产生光和氧化酶活性.
- 雌激醇对胺体的特定结合与这一过程竞争,使检测成为可能.
主要成果:
- 双模式方法实现了0.15μM (光) 和0.27μM (色度) 的检测极限.
- 该试验表明对雌激醇具有良好的选择性.
- 在肉类食品样本中成功检测出雌激素,这表明了实际应用.
结论:
- 开发的双模式检测方法提供了快速,灵敏和可靠的雌激醇检测方法.
- 这种方法在监测食品中的内分泌干扰化学物质方面具有重大潜力.
- 多模式策略提高了对食品安全应用的测试稳定性和准确性.
更多相关视频
09:07Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
6.4K
07:13Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
Published on: June 28, 2024
1.3K
相关概念视频
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
01:33Enzyme-Linked Immunosorbent Assay
13.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
13.3K