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吸入器类固醇使用改变了喘患者的口腔和呼吸道细菌和真菌微生物组概况
Kıvılcım Oğuzülgen1, Ayse Bilge Öztürk2, Ayşe Bacceoğlu3
1Department of Chest Diseases, Gazi University Faculty of Medicine, Ankara, Turkey.
International archives of allergy and immunology
|October 16, 2023
概括
与健康个体相比,喘患者表现出明显的口腔和呼吸道细菌和真菌微生物组. 吸入性皮质类固醇使用在喘患者中显著改变了这些微生物群落.
科学领域:
- 微生物学 微生物学
- 肺部病理学 肺部病理学
- 基因组学就是基因组学.
背景情况:
- 在成人喘中,细菌和真菌物种的完整谱系,特别是关于吸入性皮质类固醇 (ICS) 的使用,仍然不完全理解.
- 鉴定喘中口腔和呼吸道微生物群的特征对于了解疾病的发病和治疗效果至关重要.
研究的目的:
- 分析成人喘患者口腔和呼吸道中的细菌和真菌微生物群.
- 研究吸入性皮质类固醇 (ICS) 治疗对这些微生物群落的影响.
- 为了比较未使用类固醇的喘患者,ICS治疗的喘患者和健康对照患者之间的微生物特征.
主要方法:
- 收集了28名ICS治疗的喘患者,25名未服用类固醇的喘患者和24名健康对照者的口水和诱导液样本.
- 从所有样本中分离基因组DNA进行分析.
- 用于细菌分析的16S rRNA原料和用于真菌分析的5.8S rRNA原料.
主要成果:
- 与对照人群相比,在喘患者中发现了不同的细菌系 (例如,Neisseria,Haemophilus,Rothia在口服中;Actinomyces,Prevotella在唾液中).
- 像Malassezia restricta这样的真菌物种在喘患者的呼吸道中普遍存在.
- ICS治疗与口腔 (例如,Veillonella) 和呼吸道 (例如,Porphyromonas) 微生物群组成的变化有关.
结论:
- 在健康人群和喘患者之间,口腔和呼吸道微生物组存在显著差异.
- 吸入性皮质类固醇治疗明显影响了喘患者口腔和呼吸道微生物群落的组成.
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