一个全表观基因组关联研究儿童食欲特征和DNA甲基化
Holly A Harris1, Chloe Friedman2, Anne P Starling3
1Department of Child & Adolescent Psychiatry/Psychology, Erasmus MC, University Medical Center, Rotterdam, the Netherlands; The Generation R Study Group, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands; Erasmus University Rotterdam, Department of Psychology, Education & Child Studies, Rotterdam, the Netherlands.
Appetite
|October 16, 2023
概括
新生儿早期的DNA甲基化 (DNAm) 与儿童的食欲特征有关. 这项全表观基因组研究发现,差异甲基化区域与食物反应性和混性等行为之间存在显著的关联.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展生物学 发展生物学
- 儿科 儿科 儿科
- 营养科学 营养科学
背景情况:
- 儿童的食欲特征具有复杂的起源,早期的表观遗传修饰可能会影响食欲调节.
- 基因甲基化 (DNAm),一种表观遗传过程,涉及在CpG位点向DNA添加甲基,并可能在编程食欲方面发挥作用.
- 了解食欲调节的分子基础对于解决儿童饮食行为至关重要.
研究的目的:
- 为了研究带血DNA甲基化与儿童早期食欲特征之间的关联.
- 对全表观基因组关联研究 (EWAS) 进行元分析,检查新生儿的DNAm模式和随后的饮食行为.
- 探索生命早期的DNAm是否有助于发展特定的儿童食欲特征.
主要方法:
- 两个独立的队列 (R世代研究和健康开始研究) 的元分析,包括1,322名参与者.
- 使用Illumina Infinium HumanMethylation450 BeadChip测量带血中自体位的DNA甲基化.
- 评估儿童的食欲特征 (食物反应,情绪低食,腹反应,食物焦虑) 在4-5岁使用儿童饮食行为问卷.
- 用多重回归模型对单个CpG位点和差异甲基化区域 (DMR) 进行分析,对共变量和邦费罗尼校正进行调整.
主要成果:
- 在个体CpG位点DNA甲基化和童年食欲特征之间没有发现显著的关联.
- 对差异甲基化区域 (DMRs) 的分析揭示了显著的关联:45与食物反应,7与情绪不良饮食,13与腹反应,和9与食物焦虑.
- 这些发现表明,新生儿的DNAm模式部分与早期儿童食欲特征的变化有关.
结论:
- 新生儿DNA甲基化模式,特别是在不同甲基化的区域内,可能部分解释了儿童食欲特征的变化.
- 这项研究提供了初步证据,支持生命早期表观遗传编程,特别是DNAm在儿童食欲调节的发展中的作用.
- 进一步调查与食欲特征相关的差异性DNA甲基化是有必要的,以阐明潜在的生物学途径.
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