脱化模块对于氧化应激反应和Candida glabrata中的生物膜形成至关重要
Yue-Han Huang1, Yi-Hang Lee1, Chi-Jan Lin2
1Department of Plant Pathology and Microbiology, National Taiwan University, 10617 Taipei, Taiwan.
Medical mycology
|October 16, 2023
概括
坎迪达格拉布拉塔双化 (DUB) 模块调节真菌应激反应,生物膜形成和药物耐受性. 在DUB模块中的特定基因具有不同的作用,在宿主感染中不同影响病毒性.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 虫 (Candida glabrata) 是一种机会性真菌病原体,由于耐药性和药物坚持,导致感染增加.
- 该Spt-Ada-Gcn5乙转移酶复合体,与基因素乙化 (HAT) 和双化 (DUB) 模块,调节基因表达.
- 之前的研究将HAT模块组件ADA2与C. glabrata的应激耐受性,耐药性,细胞壁完整性和毒性联系起来.
研究的目的:
- 研究UBP8,SGF11,SGF73和SUS1基因在C. glabrata的DUB模块中的作用.
- 确定DUB模块对氧化应激耐受性,药物耐受性,细胞壁完整性和毒性的影响.
主要方法:
- 对DUB模块组件的基因表达分析.
- 对DUB模块突变 (ubp8, sgf11, sgf73, sus1) 的表型分析,以应对各种压力.
- 对生物膜形成能力的评估.
- 在小鼠系统性感染模型中对毒性的评估.
主要成果:
- UBP8,SGF11和SUS1积极调节素H2B二维基因化 (DUB).
- 缺乏UBP8,SGF11或SUS1的突变体显示生物膜形成减少,对二甲基硫酸盐和B氨基的敏感性增加.
- sgf73突变体表现出增加的生物膜形成,但易受氧化应激,抗真菌药物和细胞壁剂的影响.
- ubp8,sgf11和sus1突变显示了边际的低毒性,而sgf73显示了野生类型的毒性.
结论:
- 在C. glabrata的DUB模块在H2B无处不在,氧化应激反应,生物膜形成,细胞壁完整性和药物耐受性方面发挥着独特的作用.
- 虽然对各种细胞过程至关重要,但DUB模块在C. glabrata的毒性中表现出较小的作用.
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