相关实验视频
Updated: Jul 13, 2025

10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
24.4K
监管控制复制基因表达在纤维发育过程中,在全四棉的监管控制
Jiaqi You1, Zhenping Liu1, Zhengyang Qi1
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Nature genetics
|October 16, 2023
概括
棉花 (Gossypium hirsutum) 中的多重积分症使基因调节复杂化. 这项研究揭示了控制纤维发育的遗传模块,并突出了通过基因组启用育种策略改善纤维质量的机会.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 在理解转录调节和表型多样性方面,多重积分症带来了挑战.
- 多倍体体中亚基因组之间的基因表达的遗传调节尚未得到充分理解.
研究的目的:
- 为了研究基因调节的纤维发育在allotetraploid棉花 (Gossypium hirsutum).
- 鉴定基因模块及其在亚基因组重复基因 (同源学) 中的分区表达的特征.
- 确定基因组启用育种策略的潜在目标,以提高纤维质量.
主要方法:
- 测序了376个棉花基因组.
- 分析了2,215个时间序列转录组.
- 鉴定遗传模块和同质体表达模式.
主要成果:
- 在36个控制纤维发育的遗传模块中对1,258个基因的表征.
- 发现了控制同类动物分区表达的遗传成分.
- 鉴定了只有30%的纤维质量相关的同类素,在品种中具有有利的等位基因聚合.
结论:
- 亚基因组添加性提供了棉花纤维显著改善的潜力.
- 一个基因组启用的繁殖策略,专注于48个有利的等位基因在净化选择下,可以增强纤维表型.
- 亚基因组协调在支持诸如棉花之类的多体植物复杂表型方面发挥着至关重要的作用.
相关概念视频
Combinatorial Gene Control
8.4K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.4K
Regulation of Expression at Multiple Steps
923
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
923
Gene Duplication and Divergence
6.1K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.1K
Regulation of Expression Occurs at Multiple Steps
22.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.7K
Gene Regulation During Sporulation
20
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
20
Reporter Genes
11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K

