在Brassica juncea中,一个复杂的位点调节了高度叶状叶片的形成
Lichun Chang1, Jianli Liang1, Li Zhang1
1State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South St., Haidian, 10081, Beijing, People's Republic of China.
概括
RCO基因家族在Brassicaceae中演变为特定的血统,Brassica juncea中的两个RCO基因调节高度叶片的叶子. 这项研究阐明了RCO进化及其在作物育种叶形态学中的作用.
科学领域:
- 植物遗传学和进化研究
- 分子生物学分子生物学
- 农作物科学 农作物科学
背景情况:
- RCO基因家族调节了布拉西卡系植物中叶片状叶片的形成.
- 在Brassicaceae血统中RCO的进化历史和多样化尚未完全理解.
- 控制叶片的Brassica juncea复杂的RCO位点在很大程度上没有特征.
研究的目的:
- 为了研究RCO基因在Brassicaceae中的进化途径和多样化.
- 为了识别和表征复杂的RCO位点,负责Brassica juncea中高度叶片的叶片.
- 阐明特定RCO基因在叶子形态调节中的功能.
主要方法:
- 对16种布拉西卡系植物中55种LMI1类基因的遗传学分析.
- 合成分析将LMI1-类基因分为LMI1-类型和RCO-类型.
- 用F2种群和转录组分析对Brassica juncea中的RCO位点进行基因重复分析和映射.
主要成果:
- 在Aethionema分歧后确定了RCO的两个不同的进化路线.
- 在Brassica juncea (T84-66V2) 中发现了A10染色体上的复杂基因位点,其中有RCO和LMI1类型基因.
- 确认了BjRCO.1和BjRCO.2作为调节高度叶片叶片形成的功能基因.
结论:
- 通过重复和损失,RCO基因进化为Brassicaceae中的特定血统.
- 在B. juncea的染色体A10上的复杂位置,含有BjRCO.1和BjRCO.2,对于高度叶片的叶子发育至关重要.
- 了解RCO演变为改善布拉西卡作物的叶形态提供了洞察力.
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