相关实验视频

Updated: Jul 13, 2025

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

整合性CUT&Tag-RNA-Seq分析基因素变体宏H2A1依赖编排的人类诱导的多能干细胞重编程

Niccolò Liorni¹, Alessandro Napoli¹, Stefano Castellana¹

¹Bioinformatics Unit, Fondazione IRCCS Casa Sollievo della Sofferenza,71013, San Giovanni Rotondo, Italy.

|October 17, 2023

概括

基因素变体 macroH2A1.1 增强了人类诱导的多能干细胞 (iPSC) 重编程,比 macroH2A1.2. 这项研究使用CUT&Tag和RNA-Seq揭示了宏H2A1.1在重编程期间激活神经基因表达中的表观遗传作用.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
干细胞生物学干细胞生物学
分子生物学分子生物学

背景情况:

人类诱导的多能干细胞 (iPSC) 对再生医学至关重要,但它们的衍生效率低.
过度表达的基因组变体 macroH2A1.1 与 macroH2A1.2 不同,改善了 iPSC 重编程,尽管机制尚不清楚.

研究的目的:

调查宏H2A1.1和宏H2A1.2在编排iPSC重编程中的不同作用.
阐明基因表观机制背后的宏H2A1.1介导增强iPSC的世代.

主要方法:

在目标和标记 (CUT&Tag) 和RNA测序 (RNA-Seq) 分析下进行了整合性分离.
用人内皮细胞进行宏H2A1依赖iPSC重编程研究.
分析了全基因组占用率,转录因子结合和受宏H2A1变体调节的基因表达.

主要成果:

在重编程过程中,MacroH2A1.1表现出更广泛的基因组占用率,并与MacroH2A1.2相比调节了更多的基因.
MacroH2A1.1 特定激活的外皮和神经基因表达通路.
预测的转录因子结合部位在宏观H2A1.1相关区域中被确定.

结论:

集成的CUT&Tag和RNA-Seq是一个强大的方法来研究细胞重编程中的表观遗传机制.
宏H2A1.1在促进iPSC重编程方面发挥着重要作用,特别是通过激活神经谱系特定的基因.
了解macroH2A1.1的功能为提高iPSC发电效率提供了洞察力.

关键词:

在CUT&Tag中使用CUT&Tag.对于 iPSCs 来说,这是一个很好的选择.诱导多能干细胞的诱导干细胞.这是一个宏观的H2A1A1A1A1A1A1A1A1A1A1A.重编程是重新编程.身体细胞是体细胞的组成部分.

更多相关视频

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

相关概念视频

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...