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安德罗格拉福利德抑制传染性支气管炎病毒诱导的亡,烧亡和炎症
Jiachen Shen1, Qiuchi Xu1, Lu Chen1
1College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Antiviral therapy
|October 17, 2023
概括
安德罗格拉 (APL) 通过抑制亡,烧亡和炎症,有效地对抗禽传染性支气管炎病毒 (IBV). 这种天然化合物在保护家禽免受IBV引起的损害和降低胚胎死亡率方面表现有前途.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 禽传染性支气管炎病毒 (IBV) 对家禽业构成重大经济威胁.
- 安德罗格拉福利德 (APL) 以其抗病毒和抗炎药理学特性而闻名.
- 这项研究调查了APL减轻IBV有害影响的潜力.
研究的目的:
- 评估Andrographolide (APL) 对禽传染性支气管炎病毒 (IBV) 的抗病毒活性.
- 评估APL抑制IBV诱导的亡,亡和炎症的能力.
- 在胚胎模型中验证APL的治疗效果.
主要方法:
- 使用MTT试验评估的APL细胞毒性.
- 通过平板测试和RT-qPCR评估APL的IBV抑制.
- 在HD11细胞中通过免疫光和基因表达分析的亡,烧亡和炎症标志物.
- 在体内疗效通过胚胎试验证实.
主要成果:
- APL显著下调关键亡 (Caspase-3, -8, -9, Bax, Bid, Bak) 和亡 (DFNA5) 基因.
- APL减少了关键炎症相关基因 (NF-κB,NLRP3,iNOS,TNF-α,IL-1β) 的表达.
- APL降低了活性氧物种 (ROS) 的产生,并在胚胎试验中改善了结果,减少了死亡率和形.
结论:
- 安德罗格拉福利德 (APL) 通过抑制宿主细胞中的亡,烧亡和炎症,对IBV表现出强大的抗病毒作用.
- 这些发现在体内得到了证实,突出显示了APL对IBV的治疗潜力.
- APL为管理家禽IBV感染提供了一个有前途的天然治疗剂.
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