父亲印记的LATE-FLOWERING2转录因子有助于在小麦中对父子过度插曲的杂交障碍
Guanghui Yang1, Man Feng1, Kuohai Yu1
1State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.
Journal of integrative plant biology
|October 17, 2023
概括
在小麦中,因父交叉过多导致种子发育不良而造成的交叉杂交是具有挑战性的. 基因组印记,特别是TaLFL2基因,有助于这些障碍,解释了为什么在育种中偏爱母乳过量交叉.
科学领域:
- 植物遗传学 植物遗传学
- 农业科学 农业科学
- 进化生物学是进化的生物学.
背景情况:
- 四倍体和六倍体小麦之间的交叉体杂交在进化和繁殖中很常见.
- 六倍体小麦通常是母系父母,因为在互交交叉中种子发育不佳,但机制尚不清楚.
研究的目的:
- 为了研究在父多余交叉小麦交叉中减少种子生存能力背后的分子机制.
- 为了确定基因和流程,有助于杂交障碍在小麦 ploidy 之间.
主要方法:
- 在互交交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉交叉
- 营养代谢和印记基因的基因表达概况.
- 使用CRISPR-Cas9突变体对TaLFL2印记及其调控作用的分析.
主要成果:
- 父亲过量交叉显示,内精细胞化延迟,抑制了粉积累,减少了发芽.
- 父母的等位基因表达被扰乱,增加了印记基因,包括父亲表达的TaLFL2.
- 父亲的TaLFL2抑制了TaNAC019,抑制了父子过量交叉中的营养积累.
结论:
- 基因组印记对小麦的杂交障碍产生了重大影响.
- TaLFL2基因及其印记机制解释了在父性过量交叉中减少的种子.
- 研究结果支持在小麦育种计划中优先使用母体过剩交叉.
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