在细胞间通信中识别CD133+细胞间体,以抵消细胞内信号缺陷
Kota Kaneko1, Yan Liang1, Qing Liu1
1Department of Pathology, Department of Molecular Biology, and Moores Cancer Center, University of California at San Diego, La Jolla, United States.
eLife
|October 17, 2023
概括
CD133 (prominin 1) 是一种应激反应标记物. 信号受损会诱导CD133+囊泡,这些囊泡在细胞之间转移mRNA,有助于生存并弥补内部缺陷.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- CD133 (prominin 1) 被公认为与药物耐药性和复发相关的癌症干细胞标记物.
- 它超越癌症干细胞的作用及其精确的功能仍然是研究领域.
研究的目的:
- 研究CD133在细胞应激反应和细胞间通信中的功能.
- 阐明调节CD133表达的机制及其在补偿受损信号通路方面的作用.
主要方法:
- 使用了具有受损RTK-Shp2-Ras-Erk信号的小鼠肝脏再生模型.
- 在各种癌细胞类型中使用药物抑制增殖信号.
- 应用超分辨率显微镜,电子显微镜和单细胞RNA测序.
- 研究了肝脏和肠道有机体中的CD133缺乏细胞敏感性.
主要成果:
- 信号受损诱导了肝细胞和癌细胞中的CD133表达,表明了应激反应机制.
- CD133定位在细胞内囊泡 ("细胞间体") 中,该囊泡介导细胞间mRNA转移.
- CD133+细胞间体被mRNA丰富,促进细胞间的通信.
- 缺乏CD133的细胞对增殖信号抑制的敏感性增加.
结论:
- CD133在细胞间通信中起着至关重要的作用,通过细胞间体转移mRNA以弥补细胞内信号缺陷.
- 这种机制代表了一种固有的细胞应激反应,在整个物种中得到保护.
- 针对CD133介导的细胞间通信可能为癌症和再生医学提供新的治疗策略.
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