细胞表中的细胞相互作用 增强介质细胞 干细胞 免疫调节性质
Celia M Dunn1,2, Sumako Kameishi1, Tavie Parker1,2
1Cell Sheet Tissue Engineering Center (CSTEC), Department of Molecular Pharmaceutics, University of Utah, Salt Lake City, Utah, USA.
Tissue engineering. Part A
|October 17, 2023
概括
细胞板技术通过增加免疫调节因子和改善植入来增强治疗中细胞 (MSCs) 的作用. 这种无支架的方法通过增强的细胞相互作用来提高治疗潜力.
科学领域:
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
- 免疫学 免疫学 免疫学
背景情况:
- 介酶体 stromal 细胞 (MSCs) 在细胞疗法中用于它们的免疫调节特性,以减少炎症.
- 在MSC治疗中患者结果的变化与异质细胞的移植和保留不良有关.
- 制定改善MSC移植和治疗疗效的策略至关重要.
研究的目的:
- 通过细胞板技术生产的人类单细胞衍生克隆骨髓MSC (hcBMSC) 片,以提高免疫调节功能的潜力.
- 评估细胞板制造对关键免疫调节分子表达的影响.
- 探索细胞表基MSC治疗中细胞间相互作用的作用.
主要方法:
- 使用细胞板技术制造无脚手架的hcBMSC板.
- 细胞薄膜中的免疫调节分子表达 (IL-10, IDO-1,PTGES2) 与常规细胞悬浮的比较.
- 评估隙结抑制 (碳醇) 对细胞表中的基因表达的影响.
主要成果:
- 与细胞悬浮相比,细胞板制造在GMP级的hcBMSC线和整个MSC中显著上调免疫调节分子 (IL-10,IDO-1,PTGES2).
- 用间隙结抑制剂治疗减少了细胞表格中的IL-10和IDO-1表达,这表明细胞间通信的重要性.
- 细胞板技术有助于产生具有放大治疗因子表达的可直接转移的多细胞结构.
结论:
- 细胞板技术是一种可行的策略,可以增强MSC的免疫调节能力,用于治疗应用.
- 细胞板内的细胞间相互作用在维持和放大MSC治疗功能的过程中起着至关重要的作用.
- 这种方法提供了一种有前途的方法来克服可变性问题,并提高基于MSC的疗法的一致性.
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